BMRB Entry 50554

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50554

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Title:
C8-AcpP FabF titration
Deposition date:
2020-11-10
Original release date:
2021-05-18
Authors:
Bartholow, Thomas
Citation:

Citation: Bartholow, Thomas; Sztain, Terra; Patel, Ashay; Lee, D John; Young, Megan; Abagyan, Ruben; Burkart, Michael. "Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis"  Commun. Biol. 4, 340-340 (2021).
PubMed: 33727677

Assembly members:

Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available
entity_2, polymer, 413 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSTIEERVKKIIGEQLGVKQ EEVTNNASFVEDLGADSLDT VELVMALEEEFDTEIPDEEA EKITTVQAAIDYINGHQA
entity_2: MSKRRVVVTGLGMLSPVGNT VESTWKALLAGQSGISLIDH FDTSAYATKFAGLVKDFNCE DIISRKEQRKMDAFIQYGIV AGVQAMQDSGLEITEENATR IGAAIGSGIGGLGLIEENHT SLMNGGPRKISPFFVPSTIV NMVAGHLTIMYGLRGPSISI ATACTSGVHNIGHAARIIAY GDADVMVAGGAEKASTPLGV GGFGAARALSTRNDNPQAAS RPWDKERDGFVLGDGAGMLV LEEYEHAKKRGAKIYAELVG FGMSSDAYHMTSPPENGAGA ALAMANALRDAGIEASQIGY VNAHGTSTPAGDKAEAQAVK TIFGEAASRVLVSSTKSMTG HLLGAAGAVESIYSILALRD QAVPPTINLDNPDEGCDLDF VPHEARQVSGMEYTLCNSFG FGGTNGSLIFKKI

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AcpP1
2FabF2

Entities:

Entity 1, AcpP 78 residues - Formula weight is not available

1   METSERTHRILEGLUGLUARGVALLYSLYS
2   ILEILEGLYGLUGLNLEUGLYVALLYSGLN
3   GLUGLUVALTHRASNASNALASERPHEVAL
4   GLUASPLEUGLYALAASPSERLEUASPTHR
5   VALGLULEUVALMETALALEUGLUGLUGLU
6   PHEASPTHRGLUILEPROASPGLUGLUALA
7   GLULYSILETHRTHRVALGLNALAALAILE
8   ASPTYRILEASNGLYHISGLNALA

Entity 2, FabF 413 residues - Formula weight is not available

1   METSERLYSARGARGVALVALVALTHRGLY
2   LEUGLYMETLEUSERPROVALGLYASNTHR
3   VALGLUSERTHRTRPLYSALALEULEUALA
4   GLYGLNSERGLYILESERLEUILEASPHIS
5   PHEASPTHRSERALATYRALATHRLYSPHE
6   ALAGLYLEUVALLYSASPPHEASNCYSGLU
7   ASPILEILESERARGLYSGLUGLNARGLYS
8   METASPALAPHEILEGLNTYRGLYILEVAL
9   ALAGLYVALGLNALAMETGLNASPSERGLY
10   LEUGLUILETHRGLUGLUASNALATHRARG
11   ILEGLYALAALAILEGLYSERGLYILEGLY
12   GLYLEUGLYLEUILEGLUGLUASNHISTHR
13   SERLEUMETASNGLYGLYPROARGLYSILE
14   SERPROPHEPHEVALPROSERTHRILEVAL
15   ASNMETVALALAGLYHISLEUTHRILEMET
16   TYRGLYLEUARGGLYPROSERILESERILE
17   ALATHRALACYSTHRSERGLYVALHISASN
18   ILEGLYHISALAALAARGILEILEALATYR
19   GLYASPALAASPVALMETVALALAGLYGLY
20   ALAGLULYSALASERTHRPROLEUGLYVAL
21   GLYGLYPHEGLYALAALAARGALALEUSER
22   THRARGASNASPASNPROGLNALAALASER
23   ARGPROTRPASPLYSGLUARGASPGLYPHE
24   VALLEUGLYASPGLYALAGLYMETLEUVAL
25   LEUGLUGLUTYRGLUHISALALYSLYSARG
26   GLYALALYSILETYRALAGLULEUVALGLY
27   PHEGLYMETSERSERASPALATYRHISMET
28   THRSERPROPROGLUASNGLYALAGLYALA
29   ALALEUALAMETALAASNALALEUARGASP
30   ALAGLYILEGLUALASERGLNILEGLYTYR
31   VALASNALAHISGLYTHRSERTHRPROALA
32   GLYASPLYSALAGLUALAGLNALAVALLYS
33   THRILEPHEGLYGLUALAALASERARGVAL
34   LEUVALSERSERTHRLYSSERMETTHRGLY
35   HISLEULEUGLYALAALAGLYALAVALGLU
36   SERILETYRSERILELEUALALEUARGASP
37   GLNALAVALPROPROTHRILEASNLEUASP
38   ASNPROASPGLUGLYCYSASPLEUASPPHE
39   VALPROHISGLUALAARGGLNVALSERGLY
40   METGLUTYRTHRLEUCYSASNSERPHEGLY
41   PHEGLYGLYTHRASNGLYSERLEUILEPHE
42   LYSLYSILE

Samples:

sample_1: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.042 uM; E. coli 3-oxoacyl-[acyl-carrier-protein] synthase 2 0.0837 uM; sodium azide 0.1%; TCEP 0.5 mM; potassium phosphate 10 mM

sample_2: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.042 uM; sodium azide 0.1%; TCEP 0.5 mM; potassium phosphate 10 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC AcpP FabF titration saturatedsample_1isotropicsample_conditions_1
2D 1H-15N HSQC AcpP FabF titration zero pointsample_2isotropicsample_conditions_1
Prior assignmentsample_2isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR Pipe - processing

SPARKY - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks