BMRB Entry 50553

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50553

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Title:
15N relaxation measurements of an FG Nucleoporin in crowded conditions
Deposition date:
2020-11-09
Original release date:
2021-05-03
Authors:
Hough, Loren; Maguire, Laura; Wall, Kathryn
Citation:

Citation: Maguire, Laura; Wall, Kathryn; Hough, Loren. "Aggregation of an FG nucleoporin under crowded conditions"  .

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDNKTTNTTPSFSFGAKSDE NKAGATSKPAFSFGAKPEEK KDDNSSKPAFSFGAKSNEDK QDGTAKPAFSFGAKPAEKNN NETSKPAFSFGAKSDEKKDG DASKPAFSFGAKPDENKASA TSKPALEHHHHHH

Data typeCount
T1 relaxation values203
T2 relaxation values215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 133 residues - Formula weight is not available

1   METASPASNLYSTHRTHRASNTHRTHRPRO
2   SERPHESERPHEGLYALALYSSERASPGLU
3   ASNLYSALAGLYALATHRSERLYSPROALA
4   PHESERPHEGLYALALYSPROGLUGLULYS
5   LYSASPASPASNSERSERLYSPROALAPHE
6   SERPHEGLYALALYSSERASNGLUASPLYS
7   GLNASPGLYTHRALALYSPROALAPHESER
8   PHEGLYALALYSPROALAGLULYSASNASN
9   ASNGLUTHRSERLYSPROALAPHESERPHE
10   GLYALALYSSERASPGLULYSLYSASPGLY
11   ASPALASERLYSPROALAPHESERPHEGLY
12   ALALYSPROASPGLUASNLYSALASERALA
13   THRSERLYSPROALALEUGLUHISHISHIS
14   HISHISHIS

Samples:

sample_1: FSFG-K-6His, [U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM; PEG 13 % w/v

sample_2: FSFG-K-6His, [U-100% 13C; U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM; PVP 13 % w/v

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T2/R2 relaxationsample_1isotropicsample_conditions_1
T1/R1 relaxationsample_2isotropicsample_conditions_1

Software:

CcpNMR - NMR relaxation time constant

NMR spectrometers:

  • Varian INOVA 600 MHz