BMRB Entry 50552

Name: structure of SRSF1 RRM1 bound to RNA (AACAAA)
Published: 2020-12-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50552

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

structure of SRSF1 RRM1 bound to RNA (AACAAA)

Deposition date:

2020-11-09

Original release date:

2020-12-10

Authors:

Clery, Antoine

Citation:

Citation: Clery, Antoine; Krepl, Miroslav; Nguyen, Cristina; Moursy, Ahmed; Jorjani, Hadi; Katsantoni, Maria; Okoniewski, Michal; Mittal, Nitish; Zavolan, Mihaela; Sponer, Jiri; Allain, Frederic. "Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing" Nat. Commun. 12, 428-428 (2021).
PubMed: 33462199

Assembly members:

Assembly members:
entity_1, polymer, 6 residues, Formula weight is not available
entity_2, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AACAAA
entity_2: VIRGPAGNNDCRIYVGNLPP DIRTKDIEDVFSKYGAIRDI DLKNRRGGPPFAFVEFEDPR DAEDAVSGRDGYDYDGYRLR VEFP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	213
15N chemical shifts	74
1H chemical shifts	550

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit1	1
2	subunit2	2

Entities:

Entity 1, subunit1 6 residues - Formula weight is not available

1

A

C

A

Entity 2, subunit2 84 residues - Formula weight is not available

1

VAL

ILE

ARG

GLY

PRO

ALA

GLY

ASN

ASP

2

CYS

ARG

ILE

TYR

VAL

GLY

ASN

LEU

PRO

3

ASP

ILE

ARG

THR

LYS

ASP

ILE

GLU

ASP

VAL

4

PHE

SER

LYS

TYR

GLY

ALA

ILE

ARG

ASP

ILE

5

ASP

LEU

LYS

ASN

ARG

GLY

PRO

6

PHE

ALA

PHE

VAL

GLU

PHE

GLU

ASP

PRO

ARG

7

ASP

ALA

GLU

ASP

ALA

VAL

SER

GLY

ARG

ASP

8

GLY

TYR

ASP

TYR

ASP

GLY

TYR

ARG

LEU

ARG

9

VAL

GLU

PHE

PRO

Samples:

sample_1: RNA 0.5 mM; SRSF1 bound to RNA, [U-100% 15N], 0.5 mM

sample_2: RNA 0.5 mM; SRSF1 bound to RNA, [U-100% 13C; U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

AMBER v12 - refinement

CYANA - structure solution

TOPSPIN - collection

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz
Bruker AVANCE III 900 MHz