BMRB Entry 50545

Name: 1H, 13C and 15N NMR chemical shift assignment of A. thaliana RCD1 RST 487-589
Published: 2021-12-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50545

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N NMR chemical shift assignment of A. thaliana RCD1 RST 487-589

Deposition date:

2020-11-04

Original release date:

2021-12-15

Authors:

Staby, Lasse; Kragelund, Birthe

Citation:

Citation: Staby, Lasse; Due, Amanda; Kunze, Micha; Jorgensen, Maria; Skriver, Karen; Kragelund, Birthe. "Flanking Disorder of the Folded aa-Hub Domain from Radical Induced Cell Death1 Affects Transcription Factor Binding by Ensemble Redistribution" J. Mol. Biol. 433, 167320-167320 (2021).
PubMed: 34687712

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MARGSGSANSVGSSTTRPKS PWMPFPTLFAAISHKVAEND MLLINADYQQLRDKKMTRAE FVRKLRVIVGDDLLRSTITT LQNQPKSKEIPGSIRDHEEG AGGL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	279
15N chemical shifts	92
1H chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RCD1-RST	1

Entities:

Entity 1, RCD1-RST 104 residues - Formula weight is not available

1

MET

ALA

ARG

GLY

SER

GLY

SER

ALA

ASN

SER

2

VAL

GLY

SER

THR

ARG

PRO

LYS

SER

3

PRO

TRP

MET

PRO

PHE

PRO

THR

LEU

PHE

ALA

4

ALA

ILE

SER

HIS

LYS

VAL

ALA

GLU

ASN

ASP

5

MET

LEU

ILE

ASN

ALA

ASP

TYR

GLN

6

LEU

ARG

ASP

LYS

MET

THR

ARG

ALA

GLU

7

PHE

VAL

ARG

LYS

LEU

ARG

VAL

ILE

VAL

GLY

8

ASP

LEU

ARG

SER

THR

ILE

THR

9

LEU

GLN

ASN

GLN

PRO

LYS

SER

LYS

GLU

ILE

10

PRO

GLY

SER

ILE

ARG

ASP

HIS

GLU

GLY

11

ALA

GLY

LEU

Samples:

sample_1: RCD1-RST, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.2%; DSS 0.7 mM

sample_conditions_1: ionic strength: 143 mM; pH: 7.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D (H)N(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

qMDD - processing

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

Oxford INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks