BMRB Entry 50545

Title:
1H, 13C and 15N NMR chemical shift assignment of A. thaliana RCD1 RST 487-589
Deposition date:
2020-11-04
Original release date:
2021-12-15
Authors:
Staby, Lasse; Kragelund, Birthe
Citation:

Citation: Staby, Lasse; Due, Amanda; Kunze, Micha; Jorgensen, Maria; Skriver, Karen; Kragelund, Birthe. "Flanking Disorder of the Folded aa-Hub Domain from Radical Induced Cell Death1 Affects Transcription Factor Binding by Ensemble Redistribution"  J. Mol. Biol. 433, 167320-167320 (2021).
PubMed: 34687712

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-11a

Data sets:
Data typeCount
13C chemical shifts279
15N chemical shifts92
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RCD1-RST1

Entities:

Entity 1, RCD1-RST 104 residues - Formula weight is not available

1   METALAARGGLYSERGLYSERALAASNSER
2   VALGLYSERSERTHRTHRARGPROLYSSER
3   PROTRPMETPROPHEPROTHRLEUPHEALA
4   ALAILESERHISLYSVALALAGLUASNASP
5   METLEULEUILEASNALAASPTYRGLNGLN
6   LEUARGASPLYSLYSMETTHRARGALAGLU
7   PHEVALARGLYSLEUARGVALILEVALGLY
8   ASPASPLEULEUARGSERTHRILETHRTHR
9   LEUGLNASNGLNPROLYSSERLYSGLUILE
10   PROGLYSERILEARGASPHISGLUGLUGLY
11   ALAGLYGLYLEU

Samples:

sample_1: RCD1-RST, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.2%; DSS 0.7 mM

sample_conditions_1: ionic strength: 143 mM; pH: 7.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D (H)N(CA)NNHsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

qMDD - processing

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

  • Oxford INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks