BMRB Entry 50542

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50542

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Title:
Alpha-endosulfine (ENSA)
Deposition date:
2020-10-30
Original release date:
2021-08-10
Authors:
Thapa, Chandan; Pentikainen, Ulla; Permi, Perttu
Citation:

Citation: Thapa, Chandan; Roivas, Pekka; Haataja, Tatu; Permi, Perttu; Pentikainen, Ulla. "Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A"  FEBS J. 289, 519-534 (2022).
PubMed: 34346186

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 13000 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGTvL1-SGC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSQKQEEENPAEETGEEKQD TQEKEGILPERAEEAKLKAK YPSLGQKPGGSDFLMKRLQK GQKYFDSGDYNMAKAKMKNK QLPSAGPDKNLVTGDHIPTP QDLPQRKSSLVTSKLAGGQV E

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts119
1H chemical shifts239

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ENSA1

Entities:

Entity 1, ENSA 121 residues - 13000 Da.

1   METSERGLNLYSGLNGLUGLUGLUASNPRO
2   ALAGLUGLUTHRGLYGLUGLULYSGLNASP
3   THRGLNGLULYSGLUGLYILELEUPROGLU
4   ARGALAGLUGLUALALYSLEULYSALALYS
5   TYRPROSERLEUGLYGLNLYSPROGLYGLY
6   SERASPPHELEUMETLYSARGLEUGLNLYS
7   GLYGLNLYSTYRPHEASPSERGLYASPTYR
8   ASNMETALALYSALALYSMETLYSASNLYS
9   GLNLEUPROSERALAGLYPROASPLYSASN
10   LEUVALTHRGLYASPHISILEPROTHRPRO
11   GLNASPLEUPROGLNARGLYSSERSERLEU
12   VALTHRSERLYSLEUALAGLYGLYGLNVAL
13   GLU

Samples:

sample_1: Alpha-endosulfine (ENSA), [U-98% 13C; U-98% 15N], 0.65 mM; Ammonium Chloride, [U-99% 15N], 1 g/L; D-glucose, [U-100% 13C], 2 g/L; ammonium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; D2O, [U-99% 2H], 5%

sample_2: Alpha-endosulfine (ENSA), [U-99% 15N], 0.65 mM; Ammonium Chloride, [U-99% 15N], 1 g/L; D-glucose, [U-100% 13C], 2 g/L; ammonium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; D2O, [U-99% 2H], 5%

sample_conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D i(HCA)CO(CA)NHsample_1isotropicsample_conditions_1
3D iHA(CA)NCOsample_1isotropicsample_conditions_1
3D HA(CA)CONsample_1isotropicsample_conditions_1
3D HA(CA)CON(CA)HAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.8 - collection, processing

NMRFAM-SPARKY v1.4 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks