BMRB Entry 50539

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50539

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Title:
Mechanistic basis for ubiquitin modulation of a protein energy landscape
Deposition date:
2020-10-28
Original release date:
2021-03-17
Authors:
Carroll, Emma; Latorraca, Naomi; Lindner, Johanna; Maguire, Brendan; Pelton, Jeff; Marqusee, Susan
Citation:

Citation: Carroll, Emma; Latorraca, Naomi; Lindner, Johanna; Maguire, Brendan; Pelton, Jeff; Marqusee, Susan. "Mechanistic basis for ubiquitin modulation of a protein energy landscape"  Proc. Natl. Acad. Sci. U.S.A. 118, e2025126118-e2025126118 (2021).
PubMed: 33723075

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus amyloliquefaciens   Taxonomy ID: 1390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEC062

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGRRAVINGEQIRSISDLH QTLRRELALPEYYGENLDAL WDALTGWVEYPLVLEWRQFE QSKQLTENGAESVLQVFREA RAEGCDITIILS

Data sets:
Data typeCount
13C chemical shifts123
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BarstarK60_AC11

Entities:

Entity 1, BarstarK60_AC1 92 residues - Formula weight is not available

Six mutations compared to wildtype Barstar - K1R K2R K21R K22R C40A K78R. Cloning artifact leaves GPG scar on N-terminus.

1   GLYPROGLYARGARGALAVALILEASNGLY
2   GLUGLNILEARGSERILESERASPLEUHIS
3   GLNTHRLEUARGARGGLULEUALALEUPRO
4   GLUTYRTYRGLYGLUASNLEUASPALALEU
5   TRPASPALALEUTHRGLYTRPVALGLUTYR
6   PROLEUVALLEUGLUTRPARGGLNPHEGLU
7   GLNSERLYSGLNLEUTHRGLUASNGLYALA
8   GLUSERVALLEUGLNVALPHEARGGLUALA
9   ARGALAGLUGLYCYSASPILETHRILEILE
10   LEUSER

Related Database Links:

PDB 1BTA

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SPARKY: Backbone or all simulated peaks