BMRB Entry 50537

Name: Backbone Assignments for the Human Interleukin-17A Homodimer
Published: 2021-04-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50537

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone Assignments for the Human Interleukin-17A Homodimer

Deposition date:

2020-10-28

Original release date:

2021-04-19

Authors:

Veverka, Vaclav; Waters, Lorna; Muskett, Frederick; Taylor, Richard; Henry, Alistair; Carr, Mark

Citation:

Citation: Waters, Lorna; Veverka, Vaclav; Strong, Sarah; Muskett, Frederick; Dedi, Neesha; Lawson, Alastair; Prosser, Christine; Taylor, Richard; Henry, Alistair; Carr, Mark. "Conformational dynamics in interleukin 17A and 17F functional complexes is a key determinant of receptor A affinity and specificity" Cytokine 142, 155476-155476 (2021).
PubMed: 33706174

Assembly members:

Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GITIPRNPGCPNSEDKNFPR TVMVNLNIHNRNTNTNPKRS SDYYNRSTSPWNLHRNEDPE RYPSVIWEAKCRHLGCINAD GNVDYHMNSVPIQQEILVLR REPPHCPNSFRLEKILVSVG CTCVTPIVHHVA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	299
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IL-17A - protomer 1	1
2	IL-17A - protomer 2	1

Entities:

Entity 1, IL-17A - protomer 1 132 residues - Formula weight is not available

Mature sequence of human IL-17A

1

GLY

ILE

THR

ILE

PRO

ARG

ASN

PRO

GLY

CYS

2

PRO

ASN

SER

GLU

ASP

LYS

ASN

PHE

PRO

ARG

3

THR

VAL

MET

VAL

ASN

LEU

ASN

ILE

HIS

ASN

4

ARG

ASN

THR

ASN

THR

ASN

PRO

LYS

ARG

SER

5

SER

ASP

TYR

ASN

ARG

SER

THR

SER

PRO

6

TRP

ASN

LEU

HIS

ARG

ASN

GLU

ASP

PRO

GLU

7

ARG

TYR

PRO

SER

VAL

ILE

TRP

GLU

ALA

LYS

8

CYS

ARG

HIS

LEU

GLY

CYS

ILE

ASN

ALA

ASP

9

GLY

ASN

VAL

ASP

TYR

HIS

MET

ASN

SER

VAL

10

PRO

ILE

GLN

GLU

ILE

LEU

VAL

LEU

ARG

11

ARG

GLU

PRO

HIS

CYS

PRO

ASN

SER

PHE

12

ARG

LEU

GLU

LYS

ILE

LEU

VAL

SER

VAL

GLY

13

CYS

THR

CYS

VAL

THR

PRO

ILE

VAL

HIS

14

VAL

ALA

Samples:

sample_1: IL-17A dimer, [U-15N], 300 uM

sample_2: IL-17A dimer, [U-13C; U-15N; U-2H], 400 uM

sample_3: IL-17A dimer, [U-15N; U-2H], 450 uM

sample_conditions_1: ionic strength: 140 mM; pH: 6; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCA	sample_2	isotropic	sample_conditions_1
3D TROSY- HNCACB	sample_2	isotropic	sample_conditions_1
3D TROSY-HNCO	sample_2	isotropic	sample_conditions_1
3D TROSY-HN(CO)CACB	sample_2	isotropic	sample_conditions_1
3D 15N/1H NOESY-HSQC	sample_3	isotropic	sample_conditions_1

Software:

TOPSPIN - Data acquisition and processing

NMRPipe - Data processing

SPARKY - data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance II 800 MHz

Uniprot	Q16552
AlphaFold	Q5T2P0