BMRB Entry 5053

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PDB ID: 1ifw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5053
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Orphan PABC Domain from Saccharomyces cerevisiae Poly(A)-binding Protein

Deposition date:

2001-06-12

Original release date:

2002-08-22

Authors:

Kozlov, Guennadi; Siddiqui, Nadeem; Coillet-Matillon, Stephane; Trempe, Jean-Francois; Ekiel, Irena; Sprules, Tara; Gehring, Kalle

Citation:

Citation: Kozlov, Guennadi; Siddiqui, Nadeem; Coillet-Matillon, Stephane; Trempe, Jean-Francois; Ekiel, Irena; Sprules, Tara; Gehring, Kalle. "Solution Structure of the Orphan PABC Domain from Saccharomyces cerevisiae Poly(A)-binding Protein" J. Biol. Chem. 277, 22822-22828 (2002).

Assembly members:

Assembly members:
C-terminal domain of Pab1p, polymer, 92 residues, 10434 Da.

Natural source:

Natural source: Common Name: Yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal domain of Pab1p: GPLGSPRNANDNNQFYQQKQ RQALGEQLYKKVSAKTSNEE AAGKITGMILDLPPQEVFPL LESDELFEQHYKEASAAYES FKKEQEQQTEQA

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	504
13C chemical shifts	174
15N chemical shifts	85
coupling constants	68

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PABC domain	1

Entities:

Entity 1, PABC domain 92 residues - 10434 Da.

1

GLY

PRO

LEU

GLY

SER

PRO

ARG

ASN

ALA

ASN

2

ASP

ASN

GLN

PHE

TYR

GLN

LYS

GLN

3

ARG

GLN

ALA

LEU

GLY

GLU

GLN

LEU

TYR

LYS

4

LYS

VAL

SER

ALA

LYS

THR

SER

ASN

GLU

5

ALA

GLY

LYS

ILE

THR

GLY

MET

ILE

LEU

6

ASP

LEU

PRO

GLN

GLU

VAL

PHE

PRO

LEU

7

LEU

GLU

SER

ASP

GLU

LEU

PHE

GLU

GLN

HIS

8

TYR

LYS

GLU

ALA

SER

ALA

TYR

GLU

SER

9

PHE

LYS

GLU

GLN

GLU

GLN

THR

GLU

10

GLN

ALA

Samples:

sample_1: C-terminal domain of Pab1p, [U-15N], 3.0 mM; NaCl 150 mM

sample_2: C-terminal domain of Pab1p 3.0 mM; NaCl 150 mM

sample_3: C-terminal domain of Pab1p, [U-13C; U-15N], 3.0 mM; NaCl 150 mM

conditions_1: pH: 6.3; temperature: 303 K; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	not available	not available	not available
3D 1H-1H-15N NOESY	not available	not available	not available
3D 1H-1H-15N TOCSY	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HNCACB	not available	not available	not available

Software:

XWINNMR v2.1 - data collection

GIFA v4.31 - data processing

XEASY v1.3.13 - data analysis

ARIA v0.9 - automated NOE assignment

CNS v0.9 - refinement

NMR spectrometers:

Bruker DRX 500 MHz
Varian UnityPlus 750 MHz

PDB	1IFW
DBJ	BAA00017 GAA22994
EMBL	CAY79353
GB	AAA34787 AAA34838 AAB64692 AAT92873 AHY75721
REF	NP_011092 XP_011103263
SP	P04147
TPG	DAA07827
AlphaFold	P04147