BMRB Entry 50497

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50497

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Title:
Sarcomeric intrinsically disordered protein FATZ-1 (D91-FATZ-1)
Deposition date:
2020-10-02
Original release date:
2021-05-31
Authors:
Zawadzka-Kazimierczuk, Anna; Dubois, Pavlina; Sponga, Antonio; Arolas, Joan; Geist, Leonhard; Mlynek, Georg; Mateos, Borja; Schwarz, Thomas; Djinovic-Carugo, Kristina; Kozminski, Wiktor; Konrat, Robert
Citation:

Citation: Sponga, Antonio; Arolas, Joan; Schwarz, Thomas; Jeffries, Cy; Rodriguez Chamorro, Ariadna; Kostan, Julius; Ghisleni, Andrea; Drepper, Friedel; Polyansky, Anton; De Almeida Ribeiro, Euripedes; Pedron, Miriam; Zawadzka-Kazimierczuk, Anna; Mlynek, Georg; Peterbauer, Thomas; Doto, Pierantonio; Schreiner, Claudia; Hollerl, Eneda; Mateos, Borja; Geist, Leonhard; Faulkner, Georgine; Kozminski, Wiktor; Svergun, Dmitri; Warscheid, Bettina; Zagrovic, Bojan; Gautel, Mathias; Konrat, Robert; Djinovic-Carugo, Kristina. "Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with alpha-actinin."  Sci. Adv. 7, eabg7653-eabg7653 (2021).
PubMed: 34049882

Assembly members:

Assembly members:
entity_1, polymer, 224 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHHHHHHVDDDDKIVPTVG GQLGTAGQGFSYSKSNGRGG SQAGGSGSAGQYGSDQQHHL GSGSGAGGTGGPAGQAGRGG AAGTAGVGETGSGDQAGGEG KHITVFKTYISPWERAMGVD PQQKMELGIDLLAYGAKAEL PKYKSFNRTAMPYGGYEKAS KRMTFQMPKFDLGPLLSEPL VLYNQNLSNRPSFNRTPIPW LSSGEPVDYNVDIGIPLDGE TEEL

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts95
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1d91-FATZ-11

Entities:

Entity 1, d91-FATZ-1 224 residues - Formula weight is not available

1   METALAHISHISHISHISHISHISVALASP
2   ASPASPASPLYSILEVALPROTHRVALGLY
3   GLYGLNLEUGLYTHRALAGLYGLNGLYPHE
4   SERTYRSERLYSSERASNGLYARGGLYGLY
5   SERGLNALAGLYGLYSERGLYSERALAGLY
6   GLNTYRGLYSERASPGLNGLNHISHISLEU
7   GLYSERGLYSERGLYALAGLYGLYTHRGLY
8   GLYPROALAGLYGLNALAGLYARGGLYGLY
9   ALAALAGLYTHRALAGLYVALGLYGLUTHR
10   GLYSERGLYASPGLNALAGLYGLYGLUGLY
11   LYSHISILETHRVALPHELYSTHRTYRILE
12   SERPROTRPGLUARGALAMETGLYVALASP
13   PROGLNGLNLYSMETGLULEUGLYILEASP
14   LEULEUALATYRGLYALALYSALAGLULEU
15   PROLYSTYRLYSSERPHEASNARGTHRALA
16   METPROTYRGLYGLYTYRGLULYSALASER
17   LYSARGMETTHRPHEGLNMETPROLYSPHE
18   ASPLEUGLYPROLEULEUSERGLUPROLEU
19   VALLEUTYRASNGLNASNLEUSERASNARG
20   PROSERPHEASNARGTHRPROILEPROTRP
21   LEUSERSERGLYGLUPROVALASPTYRASN
22   VALASPILEGLYILEPROLEUASPGLYGLU
23   THRGLUGLULEU

Samples:

sample_1: d91-FATZ-1, [U-99% 13C; U-99% 15N], 0.25 mM; TRIS 50 mM; NaCl 150 mM; Arginine 500 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.7 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
5D HN(CA)CONHsample_1isotropicsample_conditions_1
5D (H)NCO(NCA)CONHsample_1isotropicsample_conditions_1
5DHabCabCONHsample_1isotropicsample_conditions_1

Software:

VNMRj - experiments setup and acquisition

SPARKY - spectra display, peak picking

TSAR - resonance assignment

NMR spectrometers:

  • Agilent DD2 800 MHz

Related Database Links:

UNP Q9NP98
AlphaFold Q9H1I7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks