BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5049

Title: Backbone 1H, 13C, and 15N data for the extracellular domain of human IFNAR2   PubMed: 11888273

Deposition date: 2001-06-10 Original release date: 2002-04-05

Authors: Chill, Jordan; ., Nivasch; ., .; ., .; ., .; , .

Citation: Chill, Jordan; ., Nivasch; ., .; ., .; ., .; 5049, .. "The Human Interferon Receptor: NMR-based Modeling, Mapping of the IFN-alpha2 Binding Site, and Observed Ligand-Induced Tightening"  Biochemistry 41, 3575-3585 (2002).

Assembly members:
Extracellular domain of subunit 2 of the Human receptor for type I interferons, polymer, 212 residues, 24850 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' . 'E. coli' . . . . . . . . . . . . . 'E. Coli TG1 cells   Host organism: 5049

Entity Sequences (FASTA):
Extracellular domain of subunit 2 of the Human receptor for type I interferons: SYDSPDYTDESCTFKISLRN FRSILSWELKNHSIVPTHYT LLYTIMSKPEDLKVVKNCAN TTRSFCDLTDEWRSTHEAYV TVLEGFSGNTTLFSCSHNFW LAIDMSFEPPEFEIVGFTNH INVMVKFPSIVEEELQFDLS LVIEEQSEGIVKKHKPEIKG NMSGNFTYIIDKLIPNTNYC VSVYLEHSDEQAVIKSPLKC TLLPPGQESEFS

Data sets:
Data typeCount
13C chemical shifts573
1831H chemical shifts

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IFNAR2-EC1

Entities:

Entity 1, IFNAR2-EC 212 residues - 24850 Da.

1   SER.50495.TYR.504911.
2   THR.504917.ARG.504923.
3   LEU.504929.ASN.504935.
4   THR.504941.TYR.504947.
5   PRO.504953.VAL.504959.
6   THR.504965.ASP.504971.
7   ARG.504977.TYR.504983.
8   GLY.504989.THR.504995.
9   HIS.5049101.ILE.5049107.
10   PRO.5049113.VAL.5049119.
11   ILE.5049125.PHE.5049131.
12   GLU.5049137.LEU.5049143.
13   GLU.5049149.VAL.5049155.
14   GLU.5049161.SER.5049167.
15   ILE.5049173.PRO.5049179.
16   VAL.5049185.HIS.5049191.
17   VAL.5049197.LYS.5049203.
18   PRO.5049209.PHE.

Samples:

sample_1: Extracellular domain of subunit 2 of the Human receptor for type I interferons' '[U-97% 13C; U-97% 15N] 0.25; .; . mM; .

Cond-1: pH: 8.0; 308: 1 5049

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available

Software:

AURELIA v2.8 - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
1N6V .
. Nmr Based Docking Model Of The Complex Between The Human Type I Interferon Receptor And Human Interferon Alpha-2 . . . . 99.06 . . . 5049
unnamed protein product [Homo sapiens] .
100.00 239 100.00 5.11e-122
331 100.00
3.54e-121 .
5049

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts