BMRB Entry 50478

Name: Human growth hormone receptor intracellular domain
Published: 2020-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50478

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human growth hormone receptor intracellular domain

Deposition date:

2020-09-23

Original release date:

2020-10-23

Authors:

Kragelund, Birthe; Nikolajsen, Louise; Haxholm, Gitte; Seiffert, Pernille

Citation:

Citation: Haxholm, Gitte; Nikolajsen, Louise; Olsen, Johan; Fredsted, Jacob; Larsen, Flemming; Goffin, Vincent; Pedersen, Stine; Brooks, Andrew; Waters, Michael; Kragelund, Birthe. "Intrinsically disordered cytoplasmic domains of two cytokine receptors mediate conserved interactions with membranes." Biochem. J. 468, 495-506 (2015).
PubMed: 25846210

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSKQQRIKMLILPPVPVPK IKGIDPDLLKEGKLEEVNTI LAIHDSYKPEFHSDDSWVEF IELDIDEPDEKTEESDTDRL LSSDHEKSHSNLGVKDGDSG RTS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	289
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GHR-ICD270-370	1

Entities:

Entity 1, GHR-ICD270-370 103 residues - Formula weight is not available

Residues 270 - 370 of the long isoform of GHR includes two non-native residues as remnants from cloning (G268 and S269).

1

GLY

SER

LYS

GLN

ARG

ILE

LYS

MET

2

LEU

ILE

LEU

PRO

VAL

PRO

VAL

PRO

LYS

3

ILE

LYS

GLY

ILE

ASP

PRO

ASP

LEU

LYS

4

GLU

GLY

LYS

LEU

GLU

VAL

ASN

THR

ILE

5

LEU

ALA

ILE

HIS

ASP

SER

TYR

LYS

PRO

GLU

6

PHE

HIS

SER

ASP

SER

TRP

VAL

GLU

PHE

7

ILE

GLU

LEU

ASP

ILE

ASP

GLU

PRO

ASP

GLU

8

LYS

THR

GLU

SER

ASP

THR

ASP

ARG

LEU

9

LEU

SER

ASP

HIS

GLU

LYS

SER

HIS

SER

10

ASN

LEU

GLY

VAL

LYS

ASP

GLY

ASP

SER

GLY

11

ARG

THR

SER

Samples:

sample_1: Human growth hormone receptor intracellular domain 270-370, [U-95% 13C; U-95% 15N], 500 mM; Na2HPO4/NaH2PO4 20 mM; tris(2-carboxyethyl)phosphine (TCEP) 6.5 mM; 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS) 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - analyses

NMRPipe - transformation and visualization

NMR spectrometers:

Agilent INOVA 750 MHz
Agilent INOVA 800 MHz

UNP	P10912-1
AlphaFold	Q9HCX2