BMRB Entry 50474

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50474

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Title:
Methyl assignments of Hsp90 FL AIM LV pro-R labeled bound to AMP-PNP
Deposition date:
2020-09-21
Original release date:
2020-12-16
Authors:
Lopez, Abraham; Sattler, Michael
Citation:

Citation: Biebl, Maximilian; Lopez, Abraham; Rehn, Alexandra; Freiburger, Lee; Lawatscheck, Jannis; Blank, Birgit; Sattler, Michael; Buchner, Johannes. "The co-chaperone p23 coordinates client binding and progression of the Hsp90 chaperone cycle via flexible regions"  Nat. Commun. 12, 828-828 (2021).
PubMed: 33547294

Assembly members:

Assembly members:
entity_1, polymer, 709 residues, Formula weight is not available
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, non-polymer, 506.196 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MASETFEFQAEITQLMSLII NTVYSNKEIFLRELISNASD ALDKIRYKSLSDPKQLETEP DLFIRITPKPEQKVLEIRDS GIGMTKAELINNLGTIAKSG TKAFMEALSAGADVSMIGQF GVGFYSLFLVADRVQVISKS NDDEQYIWESNAGGSFTVTL DEVNERIGRGTILRLFLKDD QLEYLEEKRIKEVIKRHSEF VAYPIQLVVTKEVEKEVPIP EEEKKDEEKKDEEKKDEDDK KPKLEEVDEEEEKKPKTKKV KEEVQEIEELNKTKPLWTRN PSDITQEEYNAFYKSISNDW EDPLYVKHFSVEGQLEFRAI LFIPKRAPFDLFESKKKKNN IKLYVRRVFITDEAEDLIPE WLSFVKGVVDSEDLPLNLSR EMLQQNKIMKVIRKNIVKKL IEAFNEIAEDSEQFEKFYSA FSKNIKLGVHEDTQNRAALA KLLRYNSTKSVDELTSLTDY VTRMPEHQKNIYYITGESLK AVEKSPFLDALKAKNFEVLF LTDPIDEYAFTQLKEFEGKT LVDITKDFELEETDEEKAER EKEIKEYEPLTKALKEILGD QVEKVVVSYKLLDAPAAIRT GQFGWSANMERIMKAQALRD SSMSSYMSSKKTFEISPKSP IIKELKKRVDEGGAQDKTVK DLTKLLYETALLTSGFSLDE PTSFASRINRLISLGLNIDE DEETETAPEASTAAPVEEVP ADTEMEEVD

Data sets:
Data typeCount
13C chemical shifts179
1H chemical shifts537

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsp901
2AMP-PNP2

Entities:

Entity 1, Hsp90 709 residues - Formula weight is not available

1   METALASERGLUTHRPHEGLUPHEGLNALA
2   GLUILETHRGLNLEUMETSERLEUILEILE
3   ASNTHRVALTYRSERASNLYSGLUILEPHE
4   LEUARGGLULEUILESERASNALASERASP
5   ALALEUASPLYSILEARGTYRLYSSERLEU
6   SERASPPROLYSGLNLEUGLUTHRGLUPRO
7   ASPLEUPHEILEARGILETHRPROLYSPRO
8   GLUGLNLYSVALLEUGLUILEARGASPSER
9   GLYILEGLYMETTHRLYSALAGLULEUILE
10   ASNASNLEUGLYTHRILEALALYSSERGLY
11   THRLYSALAPHEMETGLUALALEUSERALA
12   GLYALAASPVALSERMETILEGLYGLNPHE
13   GLYVALGLYPHETYRSERLEUPHELEUVAL
14   ALAASPARGVALGLNVALILESERLYSSER
15   ASNASPASPGLUGLNTYRILETRPGLUSER
16   ASNALAGLYGLYSERPHETHRVALTHRLEU
17   ASPGLUVALASNGLUARGILEGLYARGGLY
18   THRILELEUARGLEUPHELEULYSASPASP
19   GLNLEUGLUTYRLEUGLUGLULYSARGILE
20   LYSGLUVALILELYSARGHISSERGLUPHE
21   VALALATYRPROILEGLNLEUVALVALTHR
22   LYSGLUVALGLULYSGLUVALPROILEPRO
23   GLUGLUGLULYSLYSASPGLUGLULYSLYS
24   ASPGLUGLULYSLYSASPGLUASPASPLYS
25   LYSPROLYSLEUGLUGLUVALASPGLUGLU
26   GLUGLULYSLYSPROLYSTHRLYSLYSVAL
27   LYSGLUGLUVALGLNGLUILEGLUGLULEU
28   ASNLYSTHRLYSPROLEUTRPTHRARGASN
29   PROSERASPILETHRGLNGLUGLUTYRASN
30   ALAPHETYRLYSSERILESERASNASPTRP
31   GLUASPPROLEUTYRVALLYSHISPHESER
32   VALGLUGLYGLNLEUGLUPHEARGALAILE
33   LEUPHEILEPROLYSARGALAPROPHEASP
34   LEUPHEGLUSERLYSLYSLYSLYSASNASN
35   ILELYSLEUTYRVALARGARGVALPHEILE
36   THRASPGLUALAGLUASPLEUILEPROGLU
37   TRPLEUSERPHEVALLYSGLYVALVALASP
38   SERGLUASPLEUPROLEUASNLEUSERARG
39   GLUMETLEUGLNGLNASNLYSILEMETLYS
40   VALILEARGLYSASNILEVALLYSLYSLEU
41   ILEGLUALAPHEASNGLUILEALAGLUASP
42   SERGLUGLNPHEGLULYSPHETYRSERALA
43   PHESERLYSASNILELYSLEUGLYVALHIS
44   GLUASPTHRGLNASNARGALAALALEUALA
45   LYSLEULEUARGTYRASNSERTHRLYSSER
46   VALASPGLULEUTHRSERLEUTHRASPTYR
47   VALTHRARGMETPROGLUHISGLNLYSASN
48   ILETYRTYRILETHRGLYGLUSERLEULYS
49   ALAVALGLULYSSERPROPHELEUASPALA
50   LEULYSALALYSASNPHEGLUVALLEUPHE
51   LEUTHRASPPROILEASPGLUTYRALAPHE
52   THRGLNLEULYSGLUPHEGLUGLYLYSTHR
53   LEUVALASPILETHRLYSASPPHEGLULEU
54   GLUGLUTHRASPGLUGLULYSALAGLUARG
55   GLULYSGLUILELYSGLUTYRGLUPROLEU
56   THRLYSALALEULYSGLUILELEUGLYASP
57   GLNVALGLULYSVALVALVALSERTYRLYS
58   LEULEUASPALAPROALAALAILEARGTHR
59   GLYGLNPHEGLYTRPSERALAASNMETGLU
60   ARGILEMETLYSALAGLNALALEUARGASP
61   SERSERMETSERSERTYRMETSERSERLYS
62   LYSTHRPHEGLUILESERPROLYSSERPRO
63   ILEILELYSGLULEULYSLYSARGVALASP
64   GLUGLYGLYALAGLNASPLYSTHRVALLYS
65   ASPLEUTHRLYSLEULEUTYRGLUTHRALA
66   LEULEUTHRSERGLYPHESERLEUASPGLU
67   PROTHRSERPHEALASERARGILEASNARG
68   LEUILESERLEUGLYLEUASNILEASPGLU
69   ASPGLUGLUTHRGLUTHRALAPROGLUALA
70   SERTHRALAALAPROVALGLUGLUVALPRO
71   ALAASPTHRGLUMETGLUGLUVALASP

Entity 2, AMP-PNP - C10 H17 N6 O12 P3 - 506.196 Da.

1   ANP

Samples:

sample_1: Yeast Hsp90, [U-2H; 99%, 13CD; 1HD-Ile,Leu; 99%, U-2H; 99%, 13CE; 1HE-Met; 99%, U-2H; 99%, 13CG; 1HDG-Val; 99%, U-2H; 99%, 13CDB; 1HB-Ala; 99%] , 100 uM; AMP-PNP 5 mM; sodium chloride 100 mM; TRIS, [U-2H], 25 mM

sample_conditions_1: ionic strength: 0.1 M; pH*: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
4D CC NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMRPipe - processing

CcpNMR v2.4.2 - data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Related Database Links:

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