BMRB Entry 50473

Title:
Backbone Resonance Assignments of OmpR DNA-binding domain.
Deposition date:
2020-09-21
Original release date:
2020-12-29
Authors:
Sadotra, Sushant; Lou, Yuan-Chao; Chen, Chinpan
Citation:

Citation: Sadotra, Sushant; Lou, Yuan-Chao; Tang, Hao-Cheng; Chiu, Yi-Chih; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis for promoter DNA recognition by the response regulator OmpR"  J. Struct. Biol. 213, 107638-107638 (2020).
PubMed: 33152421

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-29b

Data sets:
Data typeCount
13C chemical shifts262
15N chemical shifts92
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OmpRc1

Entities:

Entity 1, OmpRc 103 residues - Formula weight is not available

1   VALILEALAPHEGLYLYSPHELYSLEUASN
2   LEUGLYTHRARGGLUMETPHEARGGLUASP
3   GLUPROMETPROLEUTHRSERGLYGLUPHE
4   ALAVALLEULYSALALEUVALSERHISPRO
5   ARGGLUPROLEUSERARGASPLYSLEUMET
6   ASNLEUALAARGGLYARGGLUTYRSERALA
7   METGLUARGSERILEASPVALGLNILESER
8   ARGLEUARGARGMETVALGLUGLUASPPRO
9   ALAHISPROARGTYRILEGLNTHRVALTRP
10   GLYLEUGLYTYRVALPHEVALPROASPGLY
11   SERLYSALA

Samples:

sample_1: OmpRc, [U-100% 15N], 1.1 mM

sample_conditions_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks