BMRB Entry 5046

Name: NMR Structure of HCV IRES RNA Domain IIIC
Published: 2005-11-11

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PDB ID: 1idv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5046
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of HCV IRES RNA Domain IIIC

Deposition date:

2003-11-21

Original release date:

2005-11-11

Authors:

Kaluarachchi, K.; Thiviyanathan, V; Rijnbrand, R.; Lemon, S.; Gorenstein, D.

Citation:

Citation: Rijnbrand, R.; Thiviyanathan, V.; Kaluarachchi, K.; Lemon, S.; Gorenstein, D.. "Mutational and structural analysis of stem-loop IIIC of the hepatitis C virus and GB virus B internal ribosome entry sites" J. Mol. Biol. 343, 805-817 (2004).
PubMed: 15476802

Assembly members:

Assembly members:
Hepatitis C Internal Ribosome Entry Site, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: 11102 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus not available

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hepatitis C Internal Ribosome Entry Site: GGGCGUGCCC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	89

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HEPATITIS C IRES RNA DOMAIN IIIC	1

Entities:

Entity 1, HEPATITIS C IRES RNA DOMAIN IIIC 10 residues - Formula weight is not available

1

G

C

G

U

G

C

Samples:

sample_1: Hepatitis C Internal Ribosome Entry Site1.0 – 2.0 mM; sodium phosphate buffer 10 mM; KCl 10 mM; EDTA 0.05 mM; H2O 90%; D2O 10%

sample_2: Hepatitis C Internal Ribosome Entry Site1.0 – 2.0 mM; sodium phosphate buffer 10 mM; KCl 10 mM; EDTA 0.05 mM; D2O 99.996%

sample_cond_1: pH: 6.8; temperature: 283 K; ionic strength: 10 mM; pressure: 1 atm

sample_cond_2: pH: 6.8; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available
2D TOCSY	not available	not available	not available
31P Hetero-TOCSY	not available	not available	not available
1H-13C HSQC	not available	not available	not available

Software:

X-PLOR v3.1 - refinement

MORASS v2.51 - refinement

NMR spectrometers:

Varian UNITYplus 600 MHz