BMRB Entry 50444

Name: Amide assignments of human GMCSF at low pH
Published: 2021-04-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50444

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Amide assignments of human GMCSF at low pH

Deposition date:

2020-08-25

Original release date:

2021-04-16

Authors:

Cui, Jennifer; Lisi, George

Citation:

Citation: Cui, Jennifer; Zhang, Fuming; Nierzwicki, Lukasz; Palermo, Giulia; Linhardt, Robert; Lisi, George. "Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor" Biochemistry 59, 3541-3553 (2020).
PubMed: 32897051

Assembly members:

Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ARSPSPSTQPWEHVNAIQEA RRLLNLSRDTAAEMNETVEV ISEMFDLQEPTCLQTRLELY KQGLRGSLTKLKGPLTMMAS HYKQHCPPTPETSCATQIIT FESFKENLKDFLLVIPFDCW EPVQE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	98
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GMCSF	1

Entities:

Entity 1, GMCSF 125 residues - Formula weight is not available

1

ALA

ARG

SER

PRO

SER

PRO

SER

THR

GLN

PRO

2

TRP

GLU

HIS

VAL

ASN

ALA

ILE

GLN

GLU

ALA

3

ARG

LEU

ASN

LEU

SER

ARG

ASP

THR

4

ALA

GLU

MET

ASN

GLU

THR

VAL

GLU

VAL

5

ILE

SER

GLU

MET

PHE

ASP

LEU

GLN

GLU

PRO

6

THR

CYS

LEU

GLN

THR

ARG

LEU

GLU

LEU

TYR

7

LYS

GLN

GLY

LEU

ARG

GLY

SER

LEU

THR

LYS

8

LEU

LYS

GLY

PRO

LEU

THR

MET

ALA

SER

9

HIS

TYR

LYS

GLN

HIS

CYS

PRO

THR

PRO

10

GLU

THR

SER

CYS

ALA

THR

GLN

ILE

THR

11

PHE

GLU

SER

PHE

LYS

GLU

ASN

LEU

LYS

ASP

12

PHE

LEU

VAL

ILE

PRO

PHE

ASP

CYS

TRP

13

GLU

PRO

VAL

GLN

GLU

Samples:

sample_1: GMCSF, [U-100% 15N], 300 uM; HEPES 20 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks