BMRB Entry 5044

Title:
1H, 13C, and 15N Chemical shift assignments for the third Immunoglobulin domain from the neural cell adhesion molecule, N-CAM
Deposition date:
2001-06-05
Original release date:
2001-11-14
Authors:
Atkins, A.; Chung, J.; Deechongkit, S.; Little, E.; Edelman, G.; Wright, P.; Cunningham, B.; Dyson, H.
Citation:

Citation: Atkins, A.; Chung, J.; Deechongkit, S.; Little, E.; Edelman, G.; Wright, P.; Cunningham, B.; Dyson, H.. "Solution Structure of the Third Immunoglobulin Domain of the Neural Cell Adhesion Molecule N-CAM: Can Solution Studies Define the Mechanism of Homophilic Binding?"  J. Mol. Biol. 311, 161-172 (2001).
PubMed: 11469865

Assembly members:

Assembly members:
Third Immunoglobulin domain of the Neural cell adhesion molecule, polymer, 107 residues, 11839 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Third Immunoglobulin domain of the Neural cell adhesion molecule: GKDIQVIVNVPPSVRARQST MNATANLSQSVTLACDADGF PEPTMTWTKDGEPIEQEDNE EKYSFNYDGSELIIKKVDKS DEAEYICIAENKAGEQDATI HLKVFAK

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts111
1H chemical shifts721

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IgIII1

Entities:

Entity 1, IgIII 107 residues - 11839 Da.

1   GLYLYSASPILEGLNVALILEVALASNVAL
2   PROPROSERVALARGALAARGGLNSERTHR
3   METASNALATHRALAASNLEUSERGLNSER
4   VALTHRLEUALACYSASPALAASPGLYPHE
5   PROGLUPROTHRMETTHRTRPTHRLYSASP
6   GLYGLUPROILEGLUGLNGLUASPASNGLU
7   GLULYSTYRSERPHEASNTYRASPGLYSER
8   GLULEUILEILELYSLYSVALASPLYSSER
9   ASPGLUALAGLUTYRILECYSILEALAGLU
10   ASNLYSALAGLYGLUGLNASPALATHRILE
11   HISLEULYSVALPHEALALYS

Samples:

sample_1: Third Immunoglobulin domain of the Neural cell adhesion molecule, [U-15N; U-13C], 1.0 mM

sample_cond_1: ionic strength: 0 mM; pH*: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated NOESYsample_1not availablesample_cond_1
3D_15N-separated NOESYsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1
CGCNsample_1not availablesample_cond_1
CGCOsample_1not availablesample_cond_1

Software:

NMRPipe v1.8 - processing

DYANA v1.5 - structure calculations

AMBER v7 - structure refinement

NMRView v3 - data analysis

SANE v1 - noe assignments

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
REF XP_010213927

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks