BMRB Entry 50426

Title:
FOXP2_FH_504-594
Deposition date:
2020-08-06
Original release date:
2021-06-30
Authors:
Richter, Gesa; Madl, Tobias
Citation:

Citation: Richter, Gesa; Gui, Tianshu; Bourgeois, Benjamin; Koyani, Chintan; Ulz, Peter; Heitzer, Ellen; von Lewinski, Dirk; Burgering, Boudewijn; Malle, Ernst; Madl, Tobias. "b-catenin regulates FOXP2 transcriptional activity via multiple binding sites"  FEBS J. 288, 3261-3284 (2021).
PubMed: 33284517

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: petZ2_1a

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts80
1H chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FOXP2_FH_504-5941

Entities:

Entity 1, FOXP2_FH_504-594 91 residues - Formula weight is not available

1   ARGPROPROPHETHRTYRALATHRLEUILE
2   ARGGLNALAILEMETGLUSERSERASPARG
3   GLNLEUTHRLEUASNGLUILETYRSERTRP
4   PHETHRARGTHRPHEALATYRPHEARGARG
5   ASNALAALATHRTRPLYSASNALAVALARG
6   HISASNLEUSERLEUHISLYSCYSPHEVAL
7   ARGVALGLUASNVALLYSGLYALAVALTRP
8   THRVALASPGLUVALGLUTYRGLNLYSARG
9   ARGSERGLNLYSILETHRGLYSERPROTHR
10   LEU

Samples:

sample_1: FOXP2_FH_504-594, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM; beta-mercaptoethanol 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CcpNMR - data analysis

TOPSPIN - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks