BMRB Entry 50414

Name: A10 nanobody protein backbone assignation
Published: 2020-08-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50414

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A10 nanobody protein backbone assignation

Deposition date:

2020-07-29

Original release date:

2020-08-11

Authors:

Kovacic, Matic; Ilc, Gregor; de Marco, Ario

Citation:

Citation: Djender, Selma; Schneider, Aurelie; Beugnet, Anne; Crepin, Ronan; Desrumeaux, Klervi Even; Romani, Chiara; Moutel, Sandrine; Perez, Franck; de Marco, Ario. "Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies." Microb. Cell Fact. 13, 140-140 (2014).
PubMed: 25223348

Assembly members:

Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: llama Taxonomy ID: 9844 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lama glama

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEVQLQASGGGFVQPGGSL RLSCAASGATSNISNMGWFR QAPGKEREFVSAISRAESRP LYYADSVKGRFTISRDNSKN TVYLQMNSLRAEDTATYYCA YMPLVRHKAYWGQGTQVTVS SAAAHHHHHHGAAEPEA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	188
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nanobody	1

Entities:

Entity 1, nanobody 137 residues - Formula weight is not available

1

MET

ALA

GLU

VAL

GLN

LEU

GLN

ALA

SER

GLY

2

GLY

PHE

VAL

GLN

PRO

GLY

SER

LEU

3

ARG

LEU

SER

CYS

ALA

SER

GLY

ALA

THR

4

SER

ASN

ILE

SER

ASN

MET

GLY

TRP

PHE

ARG

5

GLN

ALA

PRO

GLY

LYS

GLU

ARG

GLU

PHE

VAL

6

SER

ALA

ILE

SER

ARG

ALA

GLU

SER

ARG

PRO

7

LEU

TYR

ALA

ASP

SER

VAL

LYS

GLY

ARG

8

PHE

THR

ILE

SER

ARG

ASP

ASN

SER

LYS

ASN

9

THR

VAL

TYR

LEU

GLN

MET

ASN

SER

LEU

ARG

10

ALA

GLU

ASP

THR

ALA

THR

TYR

CYS

ALA

11

TYR

MET

PRO

LEU

VAL

ARG

HIS

LYS

ALA

TYR

12

TRP

GLY

GLN

GLY

THR

GLN

VAL

THR

VAL

SER

13

SER

ALA

HIS

14

GLY

ALA

GLU

PRO

GLU

ALA

Samples:

sample_1: A10 nanobody, [U-99% 13C; U-99% 15N], 0.25 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; disodium phosphate 10 mM; monosodium phosphate 1.8 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1.7 - chemical shift assignment

NMRFAM-SPARKY - data analysis

VNMR - collection

NMRPipe - processing

NMR spectrometers:

Agilent Technologies VNMRS 800 MHz 18.8T MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks