BMRB Entry 50404

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50404

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Title:
NMR assignments of the FinO domain in RNA chaperone RocC (repressor of competence)
Deposition date:
2020-07-22
Original release date:
2021-06-30
Authors:
Eidelpes, Reiner; Kim, Hyeong; Glover, Mark; Tolinger, Martin
Citation:

Citation: Eidelpes, Reiner; Kim, Hyeong; Glover, Mark; Tolinger, Martin. "NMR resonance assignments of the FinO-domain of the RNA chaperone RocC."  Biomol. NMR Assign. 15, 61-64 (2021).
PubMed: 33179208

Assembly members:

Assembly members:
entity_1, polymer, 230 residues, 25773.65 Da.

Natural source:

Natural source:   Common Name: Legionella pneumophila   Taxonomy ID: 446   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Legionella pneumophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRKQALHPRTAVINKAQKNQ SKRARSDALLWLAANFPEAF DNSLRIRPLKIGIMSDILQH AEKAEQVGVSKSKLREAVVL FTRRLDYLACLKAREVRIDL HGNPVAEVTEEEAENASMKI KKRVEKSVKNARKQVNAKAA NHSYVNNQPSTVSSVKPMNS FDSHPEPLLPIYPLRSSTYA SQNVAMQSAKSPSVVVKHKA PKQYDPDAVARLKEKLGLSR KAEDKKETTE

Data sets:
Data typeCount
13C chemical shifts408
15N chemical shifts98
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Repressor of Competence RocC1

Entities:

Entity 1, Repressor of Competence RocC 230 residues - 25773.65 Da.

1   METARGLYSGLNALALEUHISPROARGTHR
2   ALAVALILEASNLYSALAGLNLYSASNGLN
3   SERLYSARGALAARGSERASPALALEULEU
4   TRPLEUALAALAASNPHEPROGLUALAPHE
5   ASPASNSERLEUARGILEARGPROLEULYS
6   ILEGLYILEMETSERASPILELEUGLNHIS
7   ALAGLULYSALAGLUGLNVALGLYVALSER
8   LYSSERLYSLEUARGGLUALAVALVALLEU
9   PHETHRARGARGLEUASPTYRLEUALACYS
10   LEULYSALAARGGLUVALARGILEASPLEU
11   HISGLYASNPROVALALAGLUVALTHRGLU
12   GLUGLUALAGLUASNALASERMETLYSILE
13   LYSLYSARGVALGLULYSSERVALLYSASN
14   ALAARGLYSGLNVALASNALALYSALAALA
15   ASNHISSERTYRVALASNASNGLNPROSER
16   THRVALSERSERVALLYSPROMETASNSER
17   PHEASPSERHISPROGLUPROLEULEUPRO
18   ILETYRPROLEUARGSERSERTHRTYRALA
19   SERGLNASNVALALAMETGLNSERALALYS
20   SERPROSERVALVALVALLYSHISLYSALA
21   PROLYSGLNTYRASPPROASPALAVALALA
22   ARGLEULYSGLULYSLEUGLYLEUSERARG
23   LYSALAGLUASPLYSLYSGLUTHRTHRGLU

Samples:

sample_1: Repressor of Competence RocC, [U-99% 13C; U-99% 15N], 500 ± 10 uM; HEPES 25 mM; NaCl 150 mM

sample_2: Repressor of Competence RocC, [U-99% 15N], 500 ± 10 uM; HEPES 25 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe v2019.154.16.56 - processing

CcpNMR v2.4 - chemical shift assignment

NMR spectrometers:

  • Agilent DD2 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks