BMRB Entry 50403

Name: hFOXO4_FHCR3
Published: 2021-08-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50403

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

hFOXO4_FHCR3

Deposition date:

2020-07-22

Original release date:

2021-08-11

Authors:

Bourgeois, Benjamin; Madl, Tobias

Citation:

Citation: Bourgeois, Benjamin; Gui, Tianshu; Hoogeboom, Diana; Hocking, Henry; Richter, Gesa; Spreitzer, Emil; Viertler, Martin; Richter, Klaus; Madl, Tobias; Burgering, Boudewijn. "Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function" Cell Rep. 36, 109446-109446 (2021).
PubMed: 34320339

Assembly members:

Assembly members:
entity_1, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAVTGPRKGGSRRNAWGNQS YAELISQAIESAPEKRLTLA QIYEWMVRTVPYFKDKGDSN SSAGWKNSIRHNLSLHSKFI KVHNEATGKSSWWMLNPEGG KSGKAPRRRAASMDSSTEAA SQDRMPQDLDLDMYMENLEC DMDNIISDLMDEGEGLDFNF EPDP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	226
15N chemical shifts	118
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human hFOXO4 FHCR3	1

Entities:

Entity 1, human hFOXO4 FHCR3 164 residues - Formula weight is not available

1

GLY

ALA

VAL

THR

GLY

PRO

ARG

LYS

GLY

2

SER

ARG

ASN

ALA

TRP

GLY

ASN

GLN

SER

3

TYR

ALA

GLU

LEU

ILE

SER

GLN

ALA

ILE

GLU

4

SER

ALA

PRO

GLU

LYS

ARG

LEU

THR

LEU

ALA

5

GLN

ILE

TYR

GLU

TRP

MET

VAL

ARG

THR

VAL

6

PRO

TYR

PHE

LYS

ASP

LYS

GLY

ASP

SER

ASN

7

SER

ALA

GLY

TRP

LYS

ASN

SER

ILE

ARG

8

HIS

ASN

LEU

SER

LEU

HIS

SER

LYS

PHE

ILE

9

LYS

VAL

HIS

ASN

GLU

ALA

THR

GLY

LYS

SER

10

SER

TRP

MET

LEU

ASN

PRO

GLU

GLY

11

LYS

SER

GLY

LYS

ALA

PRO

ARG

ALA

12

ALA

SER

MET

ASP

SER

THR

GLU

ALA

13

SER

GLN

ASP

ARG

MET

PRO

GLN

ASP

LEU

ASP

14

LEU

ASP

MET

TYR

MET

GLU

ASN

LEU

GLU

CYS

15

ASP

MET

ASP

ASN

ILE

SER

ASP

LEU

MET

16

ASP

GLU

GLY

GLU

GLY

LEU

ASP

PHE

ASN

PHE

17

GLU

PRO

ASP

PRO

Samples:

sample_1: hFOXO4_FHCR3, [U-100% 13C; U-100% 15N], 500 uM; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - processing

NMR spectrometers:

Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks