BMRB Entry 50402

Title:
hFOXO4_465-500
Deposition date:
2020-07-22
Original release date:
2021-08-11
Authors:
Bourgeois, Benjamin; Madl, Tobias
Citation:

Citation: Bourgeois, Benjamin; Gui, Tianshu; Hoogeboom, Diana; Hocking, Henry; Richter, Gesa; Spreitzer, Emil; Viertler, Martin; Richter, Klaus; Madl, Tobias; Burgering, Boudewijn. "Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function"  Cell Rep. 36, 109446-109446 (2021).
PubMed: 34320339

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETZ1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RMPQDLDLDMYMENLECDMD NIISDLMDEGEGLDFN

Data sets:
Data typeCount
13C chemical shifts63
15N chemical shifts35
1H chemical shifts35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human hFOXO4 465-5001

Entities:

Entity 1, human hFOXO4 465-500 36 residues - Formula weight is not available

1   ARGMETPROGLNASPLEUASPLEUASPMET
2   TYRMETGLUASNLEUGLUCYSASPMETASP
3   ASNILEILESERASPLEUMETASPGLUGLY
4   GLUGLYLEUASPPHEASN

Samples:

sample_1: FOXO4_465-500, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks