BMRB Entry 5040

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5040

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Title:
Backbone 1H, 15N, and 13C Resonance Assignments of Inhibitor-1--a Protein Inhibitor of Protein Phosphatase-1
Deposition date:
2001-06-05
Original release date:
2002-01-23
Authors:
Chyan, Chia-lin; Tang, Tzu-Chun; Chen, Yi-chen; Liu, Hsin-tzu; Lin, Fang-Min; Liu, Chen-Kung; Hsieh, Meng-Juei; Shiao, Ming-Shi; Huang, Hsien-bin; Lin, Ta-Hsien
Citation:

Citation: Chyan, Chia-lin; Tang, Tzu-Chun; Chen, Yi-chen; Liu, Hsin-tzu; Lin, Fang-Min; Liu, Chen-Kung; Hsieh, Meng-Juei; Shiao, Ming-Shi; Huang, Hsien-bin; Lin, Ta-Hsien. "Letter to the Editor: Backbone 1H, 15N, and 13C Resonance Assignments of Inhibitor-1--a Protein Inhibitor of Protein Phosphatase-1"  J. Biomol. NMR 21, 287-288 (2001).
PubMed: 11775747

Assembly members:

Assembly members:
protein phosphatase inhibitor 1(I29T), polymer, 171 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein phosphatase inhibitor 1(I29T): MEQDNSPRKIQFTVPLLEPH LDPEAAEQTRRRRPTPATLV LTSDQSSPEIDEDRIPNPHL KSTLAMSPRQRKKMTRITPT MKELQMMVEHHLGQQQQGEE PEGAAESTGTQESRPPGIPD TEVESRLGTSGTAKKTAECI PKTHERGSKEPSTKEPSTHI PPLDSKGANSV

Data sets:
Data typeCount
13C chemical shifts492
15N chemical shifts148
1H chemical shifts568

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1I1(I29T) mononer1

Entities:

Entity 1, I1(I29T) mononer 171 residues - Formula weight is not available

1   METGLUGLNASPASNSERPROARGLYSILE
2   GLNPHETHRVALPROLEULEUGLUPROHIS
3   LEUASPPROGLUALAALAGLUGLNTHRARG
4   ARGARGARGPROTHRPROALATHRLEUVAL
5   LEUTHRSERASPGLNSERSERPROGLUILE
6   ASPGLUASPARGILEPROASNPROHISLEU
7   LYSSERTHRLEUALAMETSERPROARGGLN
8   ARGLYSLYSMETTHRARGILETHRPROTHR
9   METLYSGLULEUGLNMETMETVALGLUHIS
10   HISLEUGLYGLNGLNGLNGLNGLYGLUGLU
11   PROGLUGLYALAALAGLUSERTHRGLYTHR
12   GLNGLUSERARGPROPROGLYILEPROASP
13   THRGLUVALGLUSERARGLEUGLYTHRSER
14   GLYTHRALALYSLYSTHRALAGLUCYSILE
15   PROLYSTHRHISGLUARGGLYSERLYSGLU
16   PROSERTHRLYSGLUPROSERTHRHISILE
17   PROPROLEUASPSERLYSGLYALAASNSER
18   VAL

Samples:

sample_1: protein phosphatase inhibitor 1(I29T), [U-13C; U-15N], 1.0 – 1.5 mM

cond_set_1: pH: 5.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available

Software:

AURELIA v2.5 -

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

DBJ BAE90004
EMBL CAG33284
GB AAB02402 AAH22470 AAL48320 AAL48321 AAV38794
REF NP_001003062 NP_001253895 NP_001271621 NP_006732 XP_003807647
SP Q13522 Q8WMS3
AlphaFold Q13522 Q8WMS3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks