BMRB Entry 50398

Name: Backbone/side_chain assignment of human FOXO4 86_207
Published: 2021-08-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50398

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone/side_chain assignment of human FOXO4 86_207

Deposition date:

2020-07-21

Original release date:

2021-08-11

Authors:

Bourgeois, Benjamin; Madl, Tobias

Citation:

Citation: Bourgeois, Benjamin; Gui, Tianshu; Hoogeboom, Diana; Hocking, Henry; Richter, Gesa; Spreitzer, Emil; Viertler, Martin; Richter, Klaus; Madl, Tobias; Burgering, Boudewijn. "Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function" Cell Rep. 36, 109446-109446 (2021).
PubMed: 34320339

Assembly members:

Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ2_1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGAVTGPRKGGSRRNAWG NQSYAELISQAIESAPEKRL TLAQIYEWMVRTVPYFKDKG DSNSSAGWKNSIRHNLSLHS KFIKVHNEATGKSSWWMLNP EGGKSGKAPRRRAASMDSSS KLLRG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	334
15N chemical shifts	123
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human FOXO4 86_207	1

Entities:

Entity 1, human FOXO4 86_207 125 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ALA

VAL

THR

GLY

PRO

ARG

2

LYS

GLY

SER

ARG

ASN

ALA

TRP

GLY

3

ASN

GLN

SER

TYR

ALA

GLU

LEU

ILE

SER

GLN

4

ALA

ILE

GLU

SER

ALA

PRO

GLU

LYS

ARG

LEU

5

THR

LEU

ALA

GLN

ILE

TYR

GLU

TRP

MET

VAL

6

ARG

THR

VAL

PRO

TYR

PHE

LYS

ASP

LYS

GLY

7

ASP

SER

ASN

SER

ALA

GLY

TRP

LYS

ASN

8

SER

ILE

ARG

HIS

ASN

LEU

SER

LEU

HIS

SER

9

LYS

PHE

ILE

LYS

VAL

HIS

ASN

GLU

ALA

THR

10

GLY

LYS

SER

TRP

MET

LEU

ASN

PRO

11

GLU

GLY

LYS

SER

GLY

LYS

ALA

PRO

ARG

12

ARG

ALA

SER

MET

ASP

SER

13

LYS

LEU

ARG

GLY

Samples:

sample_1: human FOXO4, [U-99% 13C; U-99% 15N], 500 uM; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCACONH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - processing

NMR spectrometers:

Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks