BMRB Entry 50389

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50389

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Title:
Backbone Assignments of the Rec3 domain of CRISPR-Cas9 for S. pyogenes
Deposition date:
2020-07-13
Original release date:
2020-09-17
Authors:
Skeens, Erin; East, Kyle; Lisi, George
Citation:

Citation: Skeens, Erin; East, Kyle; Lisi, George. "1H, 13C, 15N backbone resonance assignment of the recognition lobe subdomain 3 (Rec3) from Streptococcus pyogenes CRISPR-Cas9"  Biomol. NMR Assignments 15, 25-28 (2021).
PubMed: 32935194

Assembly members:

Assembly members:
entity_1, polymer, 218 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus pyogenes   Taxonomy ID: 1314   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pyogenes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNFDKNLPNEKVLPKHSLLY EYFTVYNELTKVKYVTEGMR KPAFLSGEQKKAIVDLLFKT NRKVTVKQLKEDYFKKIECF DSVEISGVEDRFNASLGTYH DLLKIIKDKDFLDNEENEDI LEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRR YTGWGRLSRKLINGIRDKQS GKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQV

Data sets:
Data typeCount
13C chemical shifts583
15N chemical shifts200
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1spyRec31

Entities:

Entity 1, spyRec3 218 residues - Formula weight is not available

1   SERASNPHEASPLYSASNLEUPROASNGLU
2   LYSVALLEUPROLYSHISSERLEULEUTYR
3   GLUTYRPHETHRVALTYRASNGLULEUTHR
4   LYSVALLYSTYRVALTHRGLUGLYMETARG
5   LYSPROALAPHELEUSERGLYGLUGLNLYS
6   LYSALAILEVALASPLEULEUPHELYSTHR
7   ASNARGLYSVALTHRVALLYSGLNLEULYS
8   GLUASPTYRPHELYSLYSILEGLUCYSPHE
9   ASPSERVALGLUILESERGLYVALGLUASP
10   ARGPHEASNALASERLEUGLYTHRTYRHIS
11   ASPLEULEULYSILEILELYSASPLYSASP
12   PHELEUASPASNGLUGLUASNGLUASPILE
13   LEUGLUASPILEVALLEUTHRLEUTHRLEU
14   PHEGLUASPARGGLUMETILEGLUGLUARG
15   LEULYSTHRTYRALAHISLEUPHEASPASP
16   LYSVALMETLYSGLNLEULYSARGARGARG
17   TYRTHRGLYTRPGLYARGLEUSERARGLYS
18   LEUILEASNGLYILEARGASPLYSGLNSER
19   GLYLYSTHRILELEUASPPHELEULYSSER
20   ASPGLYPHEALAASNARGASNPHEMETGLN
21   LEUILEHISASPASPSERLEUTHRPHELYS
22   GLUASPILEGLNLYSALAGLNVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks