BMRB Entry 50379

Title:
N SARS-CoV-2 SR-rich motif (182-197)
Deposition date:
2020-07-07
Original release date:
2021-07-27
Authors:
Ibanez de Opakua, Alain; Savastano, Adriana; Zweckstetter, Markus
Citation:

Citation: Savastano, Adriana; Ibanez de Opakua, Alain; Rankovic, Marija; Zweckstetter, Markus. "Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates"  Nat. Commun. 11, 6041-6041 (2020).
PubMed: 33247108

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XASSRSSSRSRNSSRNSX

Data sets:
Data typeCount
13C chemical shifts32
15N chemical shifts16
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SR1

Entities:

Entity 1, SR 18 residues - Formula weight is not available

1   ACEALASERSERARGSERSERSERARGSER
2   ARGASNSERSERARGASNSERNH2

Samples:

sample_1: SR 3 mM; D2O, [U-2H], 5%; sodium phosphate 50 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 0.092 M; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks