BMRB Entry 50361

Name: 1H, 13C and 15N chemical shift assignment of an intein protein from a cyanobacterium Spirulina platensis
Published: 2020-12-31

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50361

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignment of an intein protein from a cyanobacterium Spirulina platensis

Deposition date:

2020-06-25

Original release date:

2020-12-31

Authors:

Boral, Soumendu; De, Soumya

Citation:

Citation: Boral, Soumendu; Maiti, Snigdha; Basak, Aditya; Lee, Woonghee; De, Soumya. "Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing" Biochemistry 59, 4711-4724 (2020).
PubMed: 33289560

Assembly members:

Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Arthrospira platensis Taxonomy ID: 118562 Superkingdom: Bacteria Kingdom: not available Genus/species: Arthrospira platensis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28(a)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EALTGDALILSDRGWLRIDD PTLQECRVLSYNESTQQWEW QQVLRWLDQGVRETWKIKTF QTEIKCTGNHLIRTDKGWIK AANITPKMKILSPEIDAAVK TALQDVESIEKLGVNHVYDI EVEHNHNFVANGLLVHN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	594
15N chemical shifts	157
1H chemical shifts	968

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spl DnaX mini-intein	1

Entities:

Entity 1, Spl DnaX mini-intein 137 residues - Formula weight is not available

1

GLU

ALA

LEU

THR

GLY

ASP

ALA

LEU

ILE

LEU

2

SER

ASP

ARG

GLY

TRP

LEU

ARG

ILE

ASP

3

PRO

THR

LEU

GLN

GLU

CYS

ARG

VAL

LEU

SER

4

TYR

ASN

GLU

SER

THR

GLN

TRP

GLU

TRP

5

GLN

VAL

LEU

ARG

TRP

LEU

ASP

GLN

GLY

6

VAL

ARG

GLU

THR

TRP

LYS

ILE

LYS

THR

PHE

7

GLN

THR

GLU

ILE

LYS

CYS

THR

GLY

ASN

HIS

8

LEU

ILE

ARG

THR

ASP

LYS

GLY

TRP

ILE

LYS

9

ALA

ASN

ILE

THR

PRO

LYS

MET

LYS

ILE

10

LEU

SER

PRO

GLU

ILE

ASP

ALA

VAL

LYS

11

THR

ALA

LEU

GLN

ASP

VAL

GLU

SER

ILE

GLU

12

LYS

LEU

GLY

VAL

ASN

HIS

VAL

TYR

ASP

ILE

13

GLU

VAL

GLU

HIS

ASN

HIS

ASN

PHE

VAL

ALA

14

ASN

GLY

LEU

VAL

HIS

ASN

Samples:

sample_1: Spl DnaX mini-intein, [U-100% 13C; U-100% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%

sample_conditions_1: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks