BMRB Entry 5036

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PDB ID: 1i11
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5036
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5

Deposition date:

2001-03-14

Original release date:

2001-07-05

Authors:

Cary, P.; Read, C.; Davis, B.; Driscoll, P.; Crane-Robinson, C.

Citation:

Citation: Cary, P.; Read, C.; Davis, B.; Driscoll, P.; Crane-Robinson, C.. "Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5" Protein Sci. 10, 83-98 (2001).
PubMed: 11266597

Assembly members:

Assembly members:
TRANSCRIPTION FACTOR SOX-5, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TRANSCRIPTION FACTOR SOX-5: GSPHIKRPMNAFMVWAKDER RKILQAFPDMHNSNISKILG SRWKAMTNLEKQPYYEEQAR LSKQHLEKYPDYKYKPRPKR T

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	480
15N chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TRANSCRIPTION FACTOR SOX-5	1

Entities:

Entity 1, TRANSCRIPTION FACTOR SOX-5 81 residues - Formula weight is not available

1

GLY

SER

PRO

HIS

ILE

LYS

ARG

PRO

MET

ASN

2

ALA

PHE

MET

VAL

TRP

ALA

LYS

ASP

GLU

ARG

3

ARG

LYS

ILE

LEU

GLN

ALA

PHE

PRO

ASP

MET

4

HIS

ASN

SER

ASN

ILE

SER

LYS

ILE

LEU

GLY

5

SER

ARG

TRP

LYS

ALA

MET

THR

ASN

LEU

GLU

6

LYS

GLN

PRO

TYR

GLU

GLN

ALA

ARG

7

LEU

SER

LYS

GLN

HIS

LEU

GLU

LYS

TYR

PRO

8

ASP

TYR

LYS

TYR

LYS

PRO

ARG

PRO

LYS

ARG

9

THR

Samples:

sample_1: TRANSCRIPTION FACTOR SOX-5, [U-15N], 2 – 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_2: TRANSCRIPTION FACTOR SOX-5 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; D2O 100%

sample_3: TRANSCRIPTION FACTOR SOX-5, [U-15N], 1 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_4: TRANSCRIPTION FACTOR SOX-5 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.20; temperature: 298.00 K; ionic strength: 75 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H NOESY	not available	not available	not available
2D 15N-1H HMQC-J	not available	not available	not available
3D 15N-1H NOESY	not available	not available	not available

Software:

X-PLOR v3.851 - refinement

FELIX v2.30 - data analysis

nmrview v3 - data analysis

InsightII v97.0 - data analysis

NMR spectrometers:

VARIAN UNITYplus 500 MHz

PDB	1I11
DBJ	BAA32567 BAB85048 BAC05353 BAC16233 BAC16234
EMBL	CAA09269 CAA46604 CAA46608 CAA46609 CAA46613
GB	AAB49537 AAH29220 AAH35227 AAH47665 AAH60773
REF	NP_001004385 NP_001028757 NP_001076940 NP_001107031 NP_001116382
SP	B3DM43 P35710 P35711 P40647
TPG	DAA29417
AlphaFold	B3DM43 P35710 P35711 P40647