BMRB Entry 50359

Name: Backbone 1H, 13C, 15N chemical shift assignments for apo-CCH
Published: 2024-05-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50359

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical shift assignments for apo-CCH

Deposition date:

2020-06-25

Original release date:

2024-05-07

Authors:

Hoppen, Claudia; Schwarten, Melanie; Willbold, Dieter; Groth, Georg

Citation:

Citation: Hoppen, Claudia; Schwarten, Melanie; Willbold, Dieter; Groth, Georg. "Plant copper chaperones ATX1 and CCH: Similar proteins with different properties and function" Sci. Rep. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETEV16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QGHMAQTVVLKVGMSCQGCV GAVNRVLGKMEGVESFDIDI KEQKVTVKGNVEPEAVFQTV SKTGKKTSYWPVEAEAEPKA EADPKVETVTETKTEAETKT EAKVDAKADVEPKAAEAETK PSQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	341
15N chemical shifts	113
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CCH	1

Entities:

Entity 1, CCH 123 residues - Formula weight is not available

Residues 1-3 represent a non-native tag

1

GLN

GLY

HIS

MET

ALA

GLN

THR

VAL

LEU

2

LYS

VAL

GLY

MET

SER

CYS

GLN

GLY

CYS

VAL

3

GLY

ALA

VAL

ASN

ARG

VAL

LEU

GLY

LYS

MET

4

GLU

GLY

VAL

GLU

SER

PHE

ASP

ILE

ASP

ILE

5

LYS

GLU

GLN

LYS

VAL

THR

VAL

LYS

GLY

ASN

6

VAL

GLU

PRO

GLU

ALA

VAL

PHE

GLN

THR

VAL

7

SER

LYS

THR

GLY

LYS

THR

SER

TYR

TRP

8

PRO

VAL

GLU

ALA

GLU

ALA

GLU

PRO

LYS

ALA

9

GLU

ALA

ASP

PRO

LYS

VAL

GLU

THR

VAL

THR

10

GLU

THR

LYS

THR

GLU

ALA

GLU

THR

LYS

THR

11

GLU

ALA

LYS

VAL

ASP

ALA

LYS

ALA

ASP

VAL

12

GLU

PRO

LYS

ALA

GLU

ALA

GLU

THR

LYS

13

PRO

SER

GLN

Samples:

sample_1: apo-CCH, [U-13C; U-15N], 700 uM; DTT 10 mM; NaCl 50 mM; HEPES 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.25; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO+	sample_1	isotropic	sample_conditions_1
3D (H)N(COCA)NH	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
3D (H)CANCO	sample_1	isotropic	sample_conditions_1
3D (H)CANCOi	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 750 MHz
Bruker AVANCE III 800 MHz
Varian VNMRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks