BMRB Entry 50356

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50356

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C, 15N backbone and ILMVAT methyl group assignment of the C-terminal RecA and RBD domains of the DEAD-box RNA helicase DbpA from E.coli
Deposition date:
2020-06-25
Original release date:
2021-07-12
Authors:
Wurm, Jan Philip
Citation:

Citation: Wurm, Jan Philip. "Assignment of the Ile, Leu, Val, Met and Ala methyl group resonances of the DEAD-box RNA helicase DbpA from E. coli"  Biomol. NMR Assign. 15, 121-128 (2021).
PubMed: 33277687

Assembly members:

Assembly members:
entity_1, polymer, 251 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSTDALPPIEQQFYETSSK GKIPLLQRLLSLHQPSSCVV FCNTKKDCQAVCDALNEVGQ SALSLHGDLEQRDRDQTLVR FANGSARVLVATDVAARGLD IKSLELVVNFELAWDPEVHV HRIGRTARAGNSGLAISFCA PEEAQRANIISDMLQIKLNW QTPPANSSIATLEAEMATLC IDGGKKAKMRPGDVLGALTG DIGLDGADIGKIAVHPAHVY VAVRQAVAHKAWKQLQGGKI KGKTCRVRLLK

Data sets:
Data typeCount
13C chemical shifts804
15N chemical shifts251
1H chemical shifts738

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal RecA domain + RNA binding domain1

Entities:

Entity 1, C-terminal RecA domain + RNA binding domain 251 residues - Formula weight is not available

Residues 209-457 C-terminal RecA domain + RNA binding domain of E.coli DbpA

1   GLYGLYSERTHRASPALALEUPROPROILE
2   GLUGLNGLNPHETYRGLUTHRSERSERLYS
3   GLYLYSILEPROLEULEUGLNARGLEULEU
4   SERLEUHISGLNPROSERSERCYSVALVAL
5   PHECYSASNTHRLYSLYSASPCYSGLNALA
6   VALCYSASPALALEUASNGLUVALGLYGLN
7   SERALALEUSERLEUHISGLYASPLEUGLU
8   GLNARGASPARGASPGLNTHRLEUVALARG
9   PHEALAASNGLYSERALAARGVALLEUVAL
10   ALATHRASPVALALAALAARGGLYLEUASP
11   ILELYSSERLEUGLULEUVALVALASNPHE
12   GLULEUALATRPASPPROGLUVALHISVAL
13   HISARGILEGLYARGTHRALAARGALAGLY
14   ASNSERGLYLEUALAILESERPHECYSALA
15   PROGLUGLUALAGLNARGALAASNILEILE
16   SERASPMETLEUGLNILELYSLEUASNTRP
17   GLNTHRPROPROALAASNSERSERILEALA
18   THRLEUGLUALAGLUMETALATHRLEUCYS
19   ILEASPGLYGLYLYSLYSALALYSMETARG
20   PROGLYASPVALLEUGLYALALEUTHRGLY
21   ASPILEGLYLEUASPGLYALAASPILEGLY
22   LYSILEALAVALHISPROALAHISVALTYR
23   VALALAVALARGGLNALAVALALAHISLYS
24   ALATRPLYSGLNLEUGLNGLYGLYLYSILE
25   LYSGLYLYSTHRCYSARGVALARGLEULEU
26   LYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks