BMRB Entry 50351

Title:
Assignment of base 15N, 13C and 1H chemical shifts for 5_SL6
Deposition date:
2020-06-23
Original release date:
2020-07-10
Authors:
Schwalbe, Harald; Richter, Christian
Citation:

Citation: Wacker, Anna; Weigand, Julia; Akabayov, Sabine; Altincekic, Nadide; Kaur Bains, Jasleen; Banijamali, Elnaz; Binas, Oliver; Castillo-Martinez, Jesus; Cetiner, Erhan; Ceylan, Betul; Chiu, Liang-Yuan; Davila-Calderon, Jesse; De Jesus, Vanessa; Dhamotharan, Karthikeyan; Duchardt-Ferner, Elke; Ferner, Jan; Frydman, Lucio; Furtig, Boris; Gallego, Jose; Grun, J. Tassilo; Hacker, Carolin; Haddad, Christina; Hahnke, Martin; Hengesbach, Martin; Hiller, Fabian; Hohmann, Katharina; Hymon, Daniel; Jonker, Henry; Keller, Heiko; Knezic, Bozana; Landgraf, Tom; Lohr, Frank; Luo, Luke; Mertinkus, Klara; Muhs, Christina; Novakovic, Mihajlo; Oxenfarth, Andreas; Palomino-Schatzlein, Martina; Petzold, Katja; Peter, Stephen; Pyper, Dennis; Qureshi, Nusrat; Riad, Magdalena; Richter, Christian; Saxena, Krishna; Schamber, Tatjana; Scherf, Tali; Schlagnitweit, Judith; Schlundt, Andreas; Schnieders, Robbin; Schwalbe, Harald; Simba-Lahuasi, Alvaro; Sreeramulu, Sridhar; Stirnal, Elke; Sudakov, Alexey; Tants, Jan-Niklas; Tolbert, Blanton; Vogele, Jenny; Weiss, Lena; Wirmer-Bartoschek, Julia; Wirtz Martin, Maria; Wohnert, Jens; Zetzsche, Heidi. "Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy"  Nucleic Acids Res. 48, 12415-12435 (2020).
PubMed: 33167030

Assembly members:

Assembly members:
entity_1, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: reverse transcriptase

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCACGUCCAACUCAGUUUG CCUGUUUUACAGGUUCGCGA CGUGCC

Data sets:
Data typeCount
13C chemical shifts31
15N chemical shifts51
1H chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15_SL61

Entities:

Entity 1, 5_SL6 46 residues - Formula weight is not available

1   GGCACGUCCA
2   ACUCAGUUUG
3   CCUGUUUUAC
4   AGGUUCGCGA
5   CGUGCC

Samples:

sample_1: 5_SL6, [15N (AC) 15N13C (GU)], 600 uM; potassium phosphate 25 mM; KCl 50 mM

sample_2: 5_SL6, [15N (AC) 15N13C (GU)], 600 uM; potassium phosphate 25 mM; KCl 50 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQC[13C]-Aromatensample_2isotropicsample_conditions_2
HCCNH[13C]sample_2isotropicsample_conditions_2
2D DARRsample_2isotropicsample_conditions_2
HSQC[15N]-Aminosample_2isotropicsample_conditions_2
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_2
TROSY[15N]sample_2isotropicsample_conditions_2
1H-JR[15N]sample_2isotropicsample_conditions_2
HSQC[15N]-2Jsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_2
1H-ESsample_2isotropicsample_conditions_2
NOESY[15N]CPMGsample_2isotropicsample_conditions_2
NOESY[15N]-Iminosample_2isotropicsample_conditions_2
HNN-COSY[15N]sample_2isotropicsample_conditions_2
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

LOGS v2.2 - collection

SPARKY v3.114 - chemical shift assignment, peak picking

TOPSPIN v3.6.2 - collection

TOPSPIN v4.0.8 - collection

NMR spectrometers:

  • Bruker Bruker Avance neo 900 MHz 900 MHz
  • Bruker Bruker Avance III HD 700 MHz 700 MHz