BMRB Entry 50350
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50350
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Citation: Wacker, Anna; Weigand, Julia; Akabayov, Sabine; Altincekic, Nadide; Kaur Bains, Jasleen; Banijamali, Elnaz; Binas, Oliver; Castillo-Martinez, Jesus; Cetiner, Erhan; Ceylan, Betul; Chiu, Liang-Yuan; Davila-Calderon, Jesse; De Jesus, Vanessa; Dhamotharan, Karthikeyan; Duchardt-Ferner, Elke; Ferner, Jan; Frydman, Lucio; Furtig, Boris; Gallego, Jose; Grun, J. Tassilo; Hacker, Carolin; Haddad, Christina; Hahnke, Martin; Hengesbach, Martin; Hiller, Fabian; Hohmann, Katharina; Hymon, Daniel; Jonker, Henry; Keller, Heiko; Knezic, Bozana; Landgraf, Tom; Lohr, Frank; Luo, Luke; Mertinkus, Klara; Muhs, Christina; Novakovic, Mihajlo; Oxenfarth, Andreas; Palomino-Schatzlein, Martina; Petzold, Katja; Peter, Stephen; Pyper, Dennis; Qureshi, Nusrat; Riad, Magdalena; Richter, Christian; Saxena, Krishna; Schamber, Tatjana; Scherf, Tali; Schlagnitweit, Judith; Schlundt, Andreas; Schnieders, Robbin; Schwalbe, Harald; Simba-Lahuasi, Alvaro; Sreeramulu, Sridhar; Stirnal, Elke; Sudakov, Alexey; Tants, Jan-Niklas; Tolbert, Blanton; Vogele, Jenny; Weiss, Lena; Wirmer-Bartoschek, Julia; Wirtz Martin, Maria; Wohnert, Jens; Zetzsche, Heidi. "Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy" Nucleic Acids Res. 48, 12415-12435 (2020).
PubMed: 33167030
Assembly members:
entity_1, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: reverse transcriptase
Entity Sequences (FASTA):
entity_1: GGAAUUCUCGUAACUACAUA
GCACAAGUAGAUGUAGUUAA
CUUUAAUCUCACACUUCGGU
GUGAUUUUAAUAGCUUCUUA
GGAGAAUGAC
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 30 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 3_SL3base | 1 |
Entities:
Entity 1, 3_SL3base 90 residues - Formula weight is not available
| 1 | G | G | A | A | U | U | C | U | C | G | |
| 2 | U | A | A | C | U | A | C | A | U | A | |
| 3 | G | C | A | C | A | A | G | U | A | G | |
| 4 | A | U | G | U | A | G | U | U | A | A | |
| 5 | C | U | U | U | A | A | U | C | U | C | |
| 6 | A | C | A | C | U | U | C | G | G | U | |
| 7 | G | U | G | A | U | U | U | U | A | A | |
| 8 | U | A | G | C | U | U | C | U | U | A | |
| 9 | G | G | A | G | A | A | U | G | A | C |
Samples:
sample_1: 3_SL3base, [U-15N], 0.23 mM; potassium phosphate 25 mM; potassium chloride 50 mM
sample_conditions_1: ionic strength: 0 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC[15N]-2J | sample_1 | isotropic | sample_conditions_1 |
| HNN-COSY[15N] | sample_1 | isotropic | sample_conditions_1 |
| TROSY[15N] | sample_1 | isotropic | sample_conditions_1 |
| 1H-JR[15N] | sample_1 | isotropic | sample_conditions_1 |
| NOESY[15N]-Imino | sample_1 | isotropic | sample_conditions_1 |
Software:
LOGS v2.2 - collection
SPARKY v3.114 - chemical shift assignment, peak picking
TOPSPIN v4.0.9 - collection
NMR spectrometers:
- Bruker Bruker Avance neo 600 MHz 600 MHz