BMRB Entry 50338

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50338

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Title:
1H, 13C, 15N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10A from Jonesia denitrificans
Deposition date:
2020-06-23
Original release date:
2021-01-08
Authors:
Christensen, Idd; Aachmann, Finn; Courtade, Gaston
Citation:

Citation: Christensen, Idd; Eijsink, Vincent; Aachmann, Finn; Courtade, Gaston. "1H, 13C, 15N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10A from Jonesia denitrificans"  Biomol. NMR Assignments 15, 79-84 (2021).
PubMed: 33215349

Assembly members:

Assembly members:
entity_1, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Jonesia denitrificans   Taxonomy ID: 43674   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Jonesia denitrificans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJB_PmXylS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HGWVTDPPSRQALCASGETS FDCGQISYEPQSVEAPKGAT TCSGGNEAFAILDDNSKPWP TTEIASTVDLTWKLTAPHNT STWEYFVDGQLHQTFDQKGQ QPPTSLTHTLTDLPTGEHTI LARWNVSNTNNAFYNCMDVV VS

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts124
1H chemical shifts501

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1JdLPMO10A1

Entities:

Entity 1, JdLPMO10A 142 residues - Formula weight is not available

1   HISGLYTRPVALTHRASPPROPROSERARG
2   GLNALALEUCYSALASERGLYGLUTHRSER
3   PHEASPCYSGLYGLNILESERTYRGLUPRO
4   GLNSERVALGLUALAPROLYSGLYALATHR
5   THRCYSSERGLYGLYASNGLUALAPHEALA
6   ILELEUASPASPASNSERLYSPROTRPPRO
7   THRTHRGLUILEALASERTHRVALASPLEU
8   THRTRPLYSLEUTHRALAPROHISASNTHR
9   SERTHRTRPGLUTYRPHEVALASPGLYGLN
10   LEUHISGLNTHRPHEASPGLNLYSGLYGLN
11   GLNPROPROTHRSERLEUTHRHISTHRLEU
12   THRASPLEUPROTHRGLYGLUHISTHRILE
13   LEUALAARGTRPASNVALSERASNTHRASN
14   ASNALAPHETYRASNCYSMETASPVALVAL
15   VALSER

Samples:

sample_1: JdLPMO10A, [U-98% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 10 mM

sample_2: JdLPMO10A, [U-98% 13C; U-98% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 10 mM

sample_3: JdLPMO10A, [U-98% 13C; U-98% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 10 mM

sample_conditions_1: ionic strength: 15 mM; pH: 5.5; pressure: 1 atm; temperature: 298.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN v4.0.7 - collection

CARA v1.5.5 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks