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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50337
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Tonelli, Marco; Rienstra, Chad; Anderson, Thomas; Kirchdoerf, Robert; Henzler-Wildman, Katherine. "1H, 13C, and 15N backbone and side chain chemical shift assignments of the SARS-CoV-2 non-structural protein 7" Biomol. NMR Assign. 15, 73-77 (2021).
PubMed: 33219414
Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: SARS CoV2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET46
Entity Sequences (FASTA):
entity_1: GSKMSDVKCTSVVLLSVLQQ
LRVESSSKLWAQCVQLHNDI
LLAKDTTEAFEKMVSLLSVL
LSMQGAVDINKLCEEMLDNR
ATLQ
Data type | Count |
13C chemical shifts | 372 |
15N chemical shifts | 93 |
1H chemical shifts | 625 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | nsp7 | 1 |
Entity 1, nsp7 84 residues - Formula weight is not available
1 | GLY | SER | LYS | MET | SER | ASP | VAL | LYS | CYS | THR | ||||
2 | SER | VAL | VAL | LEU | LEU | SER | VAL | LEU | GLN | GLN | ||||
3 | LEU | ARG | VAL | GLU | SER | SER | SER | LYS | LEU | TRP | ||||
4 | ALA | GLN | CYS | VAL | GLN | LEU | HIS | ASN | ASP | ILE | ||||
5 | LEU | LEU | ALA | LYS | ASP | THR | THR | GLU | ALA | PHE | ||||
6 | GLU | LYS | MET | VAL | SER | LEU | LEU | SER | VAL | LEU | ||||
7 | LEU | SER | MET | GLN | GLY | ALA | VAL | ASP | ILE | ASN | ||||
8 | LYS | LEU | CYS | GLU | GLU | MET | LEU | ASP | ASN | ARG | ||||
9 | ALA | THR | LEU | GLN |
sample_1: nsp7, [U-100% 13C; U-100% 15N], 1.7 mM; MOPS 10 mM; sodium chloride 150 mM; DTT 2 mM; sodium azide 0.05%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C-HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(C)CH-TOCSY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D NOESY 1H-13C-HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C-HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
3D (HB)CB(CGCD)HE | sample_1 | isotropic | sample_conditions_1 |
3D H(C)CH-TOCSY aromatic | sample_1 | isotropic | sample_conditions_1 |
2D NOESY 1H-13C-HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
VNMRj - collection
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
I-PINE - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks