BMRB Entry 5033
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5033
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lamberty, M.; Caille, A.; Landon, C.; Tassin-Moindrot, S.; Hetru, C.; Bulet, P.; Vovelle, F.. "Solution Structures of the Antifungal Heliomicin and a Selected Variant with
both Antibacterial and Antifungal Activities" Biochemistry 40, 11995-12003 (2001).
PubMed: 11580275
Assembly members:
DEFENSIN HELIOMICIN, polymer, 44 residues, Formula weight is not available
Natural source: Common Name: Tobacco budworm Taxonomy ID: 7102 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Heliothis virescens
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae
Entity Sequences (FASTA):
DEFENSIN HELIOMICIN: DKLIGSCVWGAVNYTSDCNG
ECKRRGYKGGHCGSFANVNC
WCET
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 275 |
| coupling constants | 34 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DEFENSIN HELIOMICIN | 1 |
Entities:
Entity 1, DEFENSIN HELIOMICIN 44 residues - Formula weight is not available
| 1 | ASP | LYS | LEU | ILE | GLY | SER | CYS | VAL | TRP | GLY | ||||
| 2 | ALA | VAL | ASN | TYR | THR | SER | ASP | CYS | ASN | GLY | ||||
| 3 | GLU | CYS | LYS | ARG | ARG | GLY | TYR | LYS | GLY | GLY | ||||
| 4 | HIS | CYS | GLY | SER | PHE | ALA | ASN | VAL | ASN | CYS | ||||
| 5 | TRP | CYS | GLU | THR |
Samples:
sample_1: DEFENSIN HELIOMICIN 4 mM; sodium acetate buffer 40 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 40 mM; pH: 4.3; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H DQF-COSY | not available | not available | not available |
| 2D 1H TQF-COSY | not available | not available | not available |
| 2D 1H TOCSY | not available | not available | not available |
| 2D 1H NOESY | not available | not available | not available |
Software:
UXNMR - collection
XEASY - data analysis
DYANA - refinement
X-PLOR - refinement
NMR spectrometers:
- Bruker AMX 500 MHz