BMRB Entry 5032

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5032

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX

Deposition date:

2001-05-28

Original release date:

2001-06-05

Authors:

Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats

Citation:

Citation: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX" J. Biomol. NMR 17, 181-182 (2000).
PubMed: 10921784

Assembly members:

Assembly members:
AFX DNA binding domain, polymer, 108 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AFX DNA binding domain: MRGSHHHHHHGSRRNAWGNQ SYAELISQAIESAPEKRLTL AQIYEWMVRTVPYFKDKGDS NSSAGWKNSIRHNLSLHSKF IKVHNEATGKSSWWMLNPEG GKSGKAPR
Insulin response element from IGFBP-1 promoter: CAAGCAAAACAAACCA
Insulin response element from IGFBP-1 promoter: TGGTTTGTTTTGCTTG

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	75
13C chemical shifts	147
15N chemical shifts	75

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AFX DNA binding domain	1
2	16mer insulin response sequence, strand 1	2
3	16mer insulin response sequence, strand 2	3

Entities:

Entity 1, AFX DNA binding domain 108 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

ARG

ASN

ALA

TRP

GLY

ASN

GLN

3

SER

TYR

ALA

GLU

LEU

ILE

SER

GLN

ALA

ILE

4

GLU

SER

ALA

PRO

GLU

LYS

ARG

LEU

THR

LEU

5

ALA

GLN

ILE

TYR

GLU

TRP

MET

VAL

ARG

THR

6

VAL

PRO

TYR

PHE

LYS

ASP

LYS

GLY

ASP

SER

7

ASN

SER

ALA

GLY

TRP

LYS

ASN

SER

ILE

8

ARG

HIS

ASN

LEU

SER

LEU

HIS

SER

LYS

PHE

9

ILE

LYS

VAL

HIS

ASN

GLU

ALA

THR

GLY

LYS

10

SER

TRP

MET

LEU

ASN

PRO

GLU

GLY

11

GLY

LYS

SER

GLY

LYS

ALA

PRO

ARG

Entity 2, 16mer insulin response sequence, strand 1 16 residues - Formula weight is not available

1

DC

DA

DG

DC

DA

DC

2

DA

DC

DA

Entity 3, 16mer insulin response sequence, strand 2 16 residues - Formula weight is not available

1

DT

DG

DT

DG

DT

2

DT

DG

DC

DT

DG

Samples:

sample_1: AFX DNA binding domain, [U-13C; U-15N; U-90% 2H], 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; sodium phospate 20 mM; NaCl 50 mM; MgCl2 2 mM; NaN3 0.02 % w/v

Exp_cond: pH: 6.4; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TROSY	not available	not available	not available
1H-15N HSQC	not available	not available	not available
HNCACB-TROSY	not available	not available	not available
HNCA-TROSY	not available	not available	not available

Software:

nmrPipe - processing

ANSIG - analysis

NMR spectrometers:

Varian INOVA 800 MHz

PDB	3L2C
DBJ	BAA86199 BAE34214 BAG61928 BAG73163
EMBL	CAA63819 CAA72156
GB	AAD42108 AAH99460 AAI06762 AAI39276 AAI39305
REF	NP_001094747 NP_001100413 NP_005929 NP_061259 XP_002720130
SP	P98177 Q9WVH3
TPG	DAA12928
AlphaFold	P98177 Q9WVH3