BMRB Entry 50314

Name: FtsZ CTD (317-383)
Published: 2022-04-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50314

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

FtsZ CTD (317-383)

Deposition date:

2020-06-10

Original release date:

2022-04-13

Authors:

Perdikari, Theodora Myrto; Murthy, Anastasia; Chua, Xien; Xuan, Botai; Mathews, Kaylee; Fawzi, Nicolas; Camberg, Jodi

Citation:

Citation: Viola, Marissa; Perdikari, Theodora; Trebino, Catherine; Rahmani, Negar; Mathews, Kaylee; Pena, Carolina; Chua, XienYu; Xuan, Botai; LaBreck, Christopher; Fawzi, Nicolas; Camberg, Jodi. "An enhancer sequence in the intrinsically disordered region of FtsZ promotes polymer-guided substrate processing by ClpXP protease" Protein Sci. 31, e4306-e4306 (2022).
PubMed: 35481648

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_6xHis_GFP_TEV_FtsZ_CTD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMDKRPEITLVTNKQVQQPV MDRYQQHGMAPLTQEQKPVA KVVNDNAPQTAKEPDYLDIP AFLRKQAD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	200
15N chemical shifts	58
1H chemical shifts	58

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E. Coli FtsZ	1

Entities:

Entity 1, E. Coli FtsZ 68 residues - Formula weight is not available

1

GLY

MET

ASP

LYS

ARG

PRO

GLU

ILE

THR

LEU

2

VAL

THR

ASN

LYS

GLN

VAL

GLN

PRO

VAL

3

MET

ASP

ARG

TYR

GLN

HIS

GLY

MET

ALA

4

PRO

LEU

THR

GLN

GLU

GLN

LYS

PRO

VAL

ALA

5

LYS

VAL

ASN

ASP

ASN

ALA

PRO

GLN

THR

6

ALA

LYS

GLU

PRO

ASP

TYR

LEU

ASP

ILE

PRO

7

ALA

PHE

LEU

ARG

LYS

GLN

ALA

ASP

Samples:

sample_1: E. coli FtsZ CTD, [U-13C; U-15N], 163 uM; MES 50 mM; KCl 100 mM; MgCl2 10 mM

sample_conditions_1: ionic strength: 110 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks