BMRB Entry 5030

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PDB ID: 1tuj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5030
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignment of the Honeybee Odorant-binding Protein ASP2

Deposition date:

2001-05-23

Original release date:

2002-05-30

Authors:

Lescop, Ewen; Briand, Loic; Pernollet, Jean-Claude; van Heijenoort, Carine; Guittet, Eric

Citation:

Citation: Lescop, Ewen; Briand, Loic; Pernollet, Jean-Claude; van Heijenoort, Carine; Guittet, Eric. "Letter to the Editor: 1H, 13C and 15N Chemical Shift Assignment of the Honeybee Odorant-binding Protein ASP2" J. Biomol. NMR 21, 181-182 (2001).
PubMed: 11727984

Assembly members:

Assembly members:
ASP2, polymer, 123 residues, 13695 Da.

Natural source:

Natural source: Common Name: Honeybee Taxonomy ID: 7460 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Apis mellifera

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pNatASP2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASP2: IDQDTVVAKYMEYLMPDIMP CADELHISEDIATNIQAAKN GADMSQLGCLKACVMKRIEM LKGTELYVEPVYKMIEVVHA GNADDIQLVKGIANECIENA KGETDECNIGNKYTDCYIEK LFS

Data sets:

assigned_chemical_shifts
- ASP2_shift_set

Data type	Count
13C chemical shifts	361
15N chemical shifts	119
1H chemical shifts	466

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ASP2	1

Entities:

Entity 1, ASP2 123 residues - 13695 Da.

1

ILE

ASP

GLN

ASP

THR

VAL

ALA

LYS

TYR

2

MET

GLU

TYR

LEU

MET

PRO

ASP

ILE

MET

PRO

3

CYS

ALA

ASP

GLU

LEU

HIS

ILE

SER

GLU

ASP

4

ILE

ALA

THR

ASN

ILE

GLN

ALA

LYS

ASN

5

GLY

ALA

ASP

MET

SER

GLN

LEU

GLY

CYS

LEU

6

LYS

ALA

CYS

VAL

MET

LYS

ARG

ILE

GLU

MET

7

LEU

LYS

GLY

THR

GLU

LEU

TYR

VAL

GLU

PRO

8

VAL

TYR

LYS

MET

ILE

GLU

VAL

HIS

ALA

9

GLY

ASN

ALA

ASP

ILE

GLN

LEU

VAL

LYS

10

GLY

ILE

ALA

ASN

GLU

CYS

ILE

GLU

ASN

ALA

11

LYS

GLY

GLU

THR

ASP

GLU

CYS

ASN

ILE

GLY

12

ASN

LYS

TYR

THR

ASP

CYS

TYR

ILE

GLU

LYS

13

LEU

PHE

SER

Samples:

15N13C_H2O: ASP2, [U-98% 13C; U-98% 15N], 0.7 – 1.5 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

15N13C_D2O: ASP2, [U-98% 13C; U-98% 15N], 1.3 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; D2O 100%

15N_H2O: ASP2, [U-98% 15N], 1 mM; TSP 0.1 mM; phosphate buffer 100 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

Cond_D2O: ionic strength: 0.11 M; pH: 7.7; temperature: 308 K

Cond_H2O: ionic strength: 0.11 M; pH: 5.7; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
NOESY-HSQC	not available	not available	not available

Software:

XWINNMR v2.6 - acquisition, processing

AURELIA v2.8.4 - Analysis

NMR spectrometers:

Bruker DRX 800 MHz

PDB	1TUJ
GenBank	AAD51945 AAL60418 ABD97844
REF	NP_001011591