BMRB Entry 50298
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50298
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Marcelot, Agathe; Petitalot, Ambre; Ropars, Virginie; Le Du, Marie-Helene; Samson, Camille; Dubois, Stevens; Hoffmann, Guillaume; Miron, Simona; Cuniasse, Philippe; Marquez, Jose Antonio; Thai, Robert; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "Di-phosphorylated BAF shows altered structural dynamics and binding to DNA, but interacts with its nuclear envelope partners." Nucleic Acids Res. 49, 3841-3855 (2021).
PubMed: 33744941
Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M13
Entity Sequences (FASTA):
entity_1: GTXXQKHRDFVAEPMGEKPV
GSLAGIGEVLGKKLEERGFD
KAYVVLGQFLVLKKDEDLFR
EWLKDTAGANAKQSRDAFGA
LREWADAFL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 258 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 273 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pBAF | 1 |
Entities:
Entity 1, pBAF 89 residues - Formula weight is not available
| 1 | GLY | THR | TPO | SEP | GLN | LYS | HIS | ARG | ASP | PHE | ||||
| 2 | VAL | ALA | GLU | PRO | MET | GLY | GLU | LYS | PRO | VAL | ||||
| 3 | GLY | SER | LEU | ALA | GLY | ILE | GLY | GLU | VAL | LEU | ||||
| 4 | GLY | LYS | LYS | LEU | GLU | GLU | ARG | GLY | PHE | ASP | ||||
| 5 | LYS | ALA | TYR | VAL | VAL | LEU | GLY | GLN | PHE | LEU | ||||
| 6 | VAL | LEU | LYS | LYS | ASP | GLU | ASP | LEU | PHE | ARG | ||||
| 7 | GLU | TRP | LEU | LYS | ASP | THR | ALA | GLY | ALA | ASN | ||||
| 8 | ALA | LYS | GLN | SER | ARG | ASP | ALA | PHE | GLY | ALA | ||||
| 9 | LEU | ARG | GLU | TRP | ALA | ASP | ALA | PHE | LEU |
Samples:
sample_1: pBAF, [U-98% 15N], 350 uM; D2O 10%; DSS 1 uM; potassium phosphate 40 uM; sodium chloride 150 uM
sample_conditions_1: ionic strength: 200 mM; pH: 7.2; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
CcpNmr Analysis - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks