BMRB Entry 50275

Name: Maturation of the Functional Mouse CRES Amyloid from Globular Form
Published: 2020-06-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50275

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Maturation of the Functional Mouse CRES Amyloid from Globular Form

Deposition date:

2020-05-15

Original release date:

2020-06-08

Authors:

Hewetson, Aveline; Khan, Nazmul; Dominguez, Matthew; Do, Hoa; Kusko, Rebecca; Borcik, Collin; Rigden, Daniel; Keegan, Ronan; Sutton, Roger; Latham, Michael; Wylie, Benjamin; Cornwall, Gail

Citation:

Citation: Hewetson, Aveline; Khan, Nazmul; Dominguez, Matthew; Do, Hoa; Kusko, Rebecca; Borcik, Collin; Rigden, Daniel; Keegan, Ronan; Sutton, Roger; Latham, Michael; Wylie, Benjamin; Cornwall, Gail. "Maturation of the functional mouse CRES amyloid from globular form" Proc. Natl. Acad. Sci. U.S.A. 117, 16363-16372 (2020).
PubMed: 32601205

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-cs

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMAHMVGVDQSKNEVKAQN YFGSINISNANVKQAVWFAM KEYNKESEDKYVFLVDKILH AKLQITDRMEYQIDVQISRS NCKKPLNNTENCIPQKKPEL EKKMSCSFLVGALPWNGEFN LLSKECKDV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	534
15N chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	globular CRES	1

Entities:

Entity 1, globular CRES 129 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

HIS

MET

VAL

GLY

VAL

ASP

2

GLN

SER

LYS

ASN

GLU

VAL

LYS

ALA

GLN

ASN

3

TYR

PHE

GLY

SER

ILE

ASN

ILE

SER

ASN

ALA

4

ASN

VAL

LYS

GLN

ALA

VAL

TRP

PHE

ALA

MET

5

LYS

GLU

TYR

ASN

LYS

GLU

SER

GLU

ASP

LYS

6

TYR

VAL

PHE

LEU

VAL

ASP

LYS

ILE

LEU

HIS

7

ALA

LYS

LEU

GLN

ILE

THR

ASP

ARG

MET

GLU

8

TYR

GLN

ILE

ASP

VAL

GLN

ILE

SER

ARG

SER

9

ASN

CYS

LYS

PRO

LEU

ASN

THR

GLU

10

ASN

CYS

ILE

PRO

GLN

LYS

PRO

GLU

LEU

11

GLU

LYS

MET

SER

CYS

SER

PHE

LEU

VAL

12

GLY

ALA

LEU

PRO

TRP

ASN

GLY

GLU

PHE

ASN

13

LEU

SER

LYS

GLU

CYS

LYS

ASP

VAL

Samples:

sample_1: CRES, [U-100% 13C; U-100% 15N], 50 mM; HEPES 50 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
DARR	sample_1	isotropic	sample_conditions_1
NCACX	sample_1	isotropic	sample_conditions_1
NCOCX	sample_1	isotropic	sample_conditions_1
CANcoCX DARR	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis - chemical shift assignment

NMRPipe - processing

CS-Rosetta - structure solution

NMR spectrometers:

Varian DD2 600 MHz