BMRB Entry 50271

Name: NOP15 81-180 Y94W
Published: 2021-07-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50271

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NOP15 81-180 Y94W

Deposition date:

2020-05-12

Original release date:

2021-07-07

Authors:

Zaharias, Steve

Citation:

Citation: Zaharias, Steve; Zhang, Zihan; Davis, Kenneth; Fargason, Talia; Cashman, Derek; Yu, Tao; Zhang, Jun. "Intrinsically disordered electronegative clusters improve stability and binding specificity of RNA-binding proteins" J. Biol. Chem. 297, 100945-100945 (2021).
PubMed: 34246632

Assembly members:

Assembly members:
entity_1, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3) plyss

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVKSTSKTSTKETVTKQPTE EKPIQEKEELALETSSSSSD EEDEKDEDEIEGLAASDDEQ SGTHKIKRLNPKKQANEKKS KDKKTLEEYSGIIYVSRLPH GFHEKELSKYFAQFGDLKEV RLARNKKTGNSRHYGFLEFV NKEDAMIAQESMNNYLLMGH LLQVRVLPKGAKIEKLYKYK KRVLVEKGITKPVKQLKDNM KQKHEERIKKLAKSGIEFKW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	236
15N chemical shifts	81
1H chemical shifts	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nop15	1

Entities:

Entity 1, Nop15 220 residues - Formula weight is not available

1	MET	VAL	LYS	SER	THR	SER	LYS	THR	SER	THR
2	LYS	GLU	THR	VAL	THR	LYS	GLN	PRO	THR	GLU
3	GLU	LYS	PRO	ILE	GLN	GLU	LYS	GLU	GLU	LEU
4	ALA	LEU	GLU	THR	SER	SER	SER	SER	SER	ASP
5	GLU	GLU	ASP	GLU	LYS	ASP	GLU	ASP	GLU	ILE
6	GLU	GLY	LEU	ALA	ALA	SER	ASP	ASP	GLU	GLN
7	SER	GLY	THR	HIS	LYS	ILE	LYS	ARG	LEU	ASN
8	PRO	LYS	LYS	GLN	ALA	ASN	GLU	LYS	LYS	SER
9	LYS	ASP	LYS	LYS	THR	LEU	GLU	GLU	TYR	SER
10	GLY	ILE	ILE	TYR	VAL	SER	ARG	LEU	PRO	HIS
11	GLY	PHE	HIS	GLU	LYS	GLU	LEU	SER	LYS	TYR
12	PHE	ALA	GLN	PHE	GLY	ASP	LEU	LYS	GLU	VAL
13	ARG	LEU	ALA	ARG	ASN	LYS	LYS	THR	GLY	ASN
14	SER	ARG	HIS	TYR	GLY	PHE	LEU	GLU	PHE	VAL
15	ASN	LYS	GLU	ASP	ALA	MET	ILE	ALA	GLN	GLU
16	SER	MET	ASN	ASN	TYR	LEU	LEU	MET	GLY	HIS
17	LEU	LEU	GLN	VAL	ARG	VAL	LEU	PRO	LYS	GLY
18	ALA	LYS	ILE	GLU	LYS	LEU	TYR	LYS	TYR	LYS
19	LYS	ARG	VAL	LEU	VAL	GLU	LYS	GLY	ILE	THR
20	LYS	PRO	VAL	LYS	GLN	LEU	LYS	ASP	ASN	MET
21	LYS	GLN	LYS	HIS	GLU	GLU	ARG	ILE	LYS	LYS
22	LEU	ALA	LYS	SER	GLY	ILE	GLU	PHE	LYS	TRP

Samples:

sample_1: MES 20 mM; sodium chloride 100 mM; Arginine/Glutamic Acid 400 mM; Nop15 552 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	VAL	LYS	SER	THR	SER	LYS	THR	SER	THR
2	LYS	GLU	THR	VAL	THR	LYS	GLN	PRO	THR	GLU
3	GLU	LYS	PRO	ILE	GLN	GLU	LYS	GLU	GLU	LEU
4	ALA	LEU	GLU	THR	SER	SER	SER	SER	SER	ASP
5	GLU	GLU	ASP	GLU	LYS	ASP	GLU	ASP	GLU	ILE
6	GLU	GLY	LEU	ALA	ALA	SER	ASP	ASP	GLU	GLN
7	SER	GLY	THR	HIS	LYS	ILE	LYS	ARG	LEU	ASN
8	PRO	LYS	LYS	GLN	ALA	ASN	GLU	LYS	LYS	SER
9	LYS	ASP	LYS	LYS	THR	LEU	GLU	GLU	TYR	SER
10	GLY	ILE	ILE	TYR	VAL	SER	ARG	LEU	PRO	HIS
11	GLY	PHE	HIS	GLU	LYS	GLU	LEU	SER	LYS	TYR
12	PHE	ALA	GLN	PHE	GLY	ASP	LEU	LYS	GLU	VAL
13	ARG	LEU	ALA	ARG	ASN	LYS	LYS	THR	GLY	ASN
14	SER	ARG	HIS	TYR	GLY	PHE	LEU	GLU	PHE	VAL
15	ASN	LYS	GLU	ASP	ALA	MET	ILE	ALA	GLN	GLU
16	SER	MET	ASN	ASN	TYR	LEU	LEU	MET	GLY	HIS
17	LEU	LEU	GLN	VAL	ARG	VAL	LEU	PRO	LYS	GLY
18	ALA	LYS	ILE	GLU	LYS	LEU	TYR	LYS	TYR	LYS
19	LYS	ARG	VAL	LEU	VAL	GLU	LYS	GLY	ILE	THR
20	LYS	PRO	VAL	LYS	GLN	LEU	LYS	ASP	ASN	MET
21	LYS	GLN	LYS	HIS	GLU	GLU	ARG	ILE	LYS	LYS
22	LEU	ALA	LYS	SER	GLY	ILE	GLU	PHE	LYS	TRP

1	MET	VAL	LYS	SER	THR	SER	LYS	THR	SER	THR
2	LYS	GLU	THR	VAL	THR	LYS	GLN	PRO	THR	GLU
3	GLU	LYS	PRO	ILE	GLN	GLU	LYS	GLU	GLU	LEU
4	ALA	LEU	GLU	THR	SER	SER	SER	SER	SER	ASP
5	GLU	GLU	ASP	GLU	LYS	ASP	GLU	ASP	GLU	ILE
6	GLU	GLY	LEU	ALA	ALA	SER	ASP	ASP	GLU	GLN
7	SER	GLY	THR	HIS	LYS	ILE	LYS	ARG	LEU	ASN
8	PRO	LYS	LYS	GLN	ALA	ASN	GLU	LYS	LYS	SER
9	LYS	ASP	LYS	LYS	THR	LEU	GLU	GLU	TYR	SER
10	GLY	ILE	ILE	TYR	VAL	SER	ARG	LEU	PRO	HIS
11	GLY	PHE	HIS	GLU	LYS	GLU	LEU	SER	LYS	TYR
12	PHE	ALA	GLN	PHE	GLY	ASP	LEU	LYS	GLU	VAL
13	ARG	LEU	ALA	ARG	ASN	LYS	LYS	THR	GLY	ASN
14	SER	ARG	HIS	TYR	GLY	PHE	LEU	GLU	PHE	VAL
15	ASN	LYS	GLU	ASP	ALA	MET	ILE	ALA	GLN	GLU
16	SER	MET	ASN	ASN	TYR	LEU	LEU	MET	GLY	HIS
17	LEU	LEU	GLN	VAL	ARG	VAL	LEU	PRO	LYS	GLY
18	ALA	LYS	ILE	GLU	LYS	LEU	TYR	LYS	TYR	LYS
19	LYS	ARG	VAL	LEU	VAL	GLU	LYS	GLY	ILE	THR
20	LYS	PRO	VAL	LYS	GLN	LEU	LYS	ASP	ASN	MET
21	LYS	GLN	LYS	HIS	GLU	GLU	ARG	ILE	LYS	LYS
22	LEU	ALA	LYS	SER	GLY	ILE	GLU	PHE	LYS	TRP

1	MET	VAL	LYS	SER	THR	SER	LYS	THR	SER	THR
2	LYS	GLU	THR	VAL	THR	LYS	GLN	PRO	THR	GLU
3	GLU	LYS	PRO	ILE	GLN	GLU	LYS	GLU	GLU	LEU
4	ALA	LEU	GLU	THR	SER	SER	SER	SER	SER	ASP
5	GLU	GLU	ASP	GLU	LYS	ASP	GLU	ASP	GLU	ILE
6	GLU	GLY	LEU	ALA	ALA	SER	ASP	ASP	GLU	GLN
7	SER	GLY	THR	HIS	LYS	ILE	LYS	ARG	LEU	ASN
8	PRO	LYS	LYS	GLN	ALA	ASN	GLU	LYS	LYS	SER
9	LYS	ASP	LYS	LYS	THR	LEU	GLU	GLU	TYR	SER
10	GLY	ILE	ILE	TYR	VAL	SER	ARG	LEU	PRO	HIS
11	GLY	PHE	HIS	GLU	LYS	GLU	LEU	SER	LYS	TYR
12	PHE	ALA	GLN	PHE	GLY	ASP	LEU	LYS	GLU	VAL
13	ARG	LEU	ALA	ARG	ASN	LYS	LYS	THR	GLY	ASN
14	SER	ARG	HIS	TYR	GLY	PHE	LEU	GLU	PHE	VAL
15	ASN	LYS	GLU	ASP	ALA	MET	ILE	ALA	GLN	GLU
16	SER	MET	ASN	ASN	TYR	LEU	LEU	MET	GLY	HIS
17	LEU	LEU	GLN	VAL	ARG	VAL	LEU	PRO	LYS	GLY
18	ALA	LYS	ILE	GLU	LYS	LEU	TYR	LYS	TYR	LYS
19	LYS	ARG	VAL	LEU	VAL	GLU	LYS	GLY	ILE	THR
20	LYS	PRO	VAL	LYS	GLN	LEU	LYS	ASP	ASN	MET
21	LYS	GLN	LYS	HIS	GLU	GLU	ARG	ILE	LYS	LYS
22	LEU	ALA	LYS	SER	GLY	ILE	GLU	PHE	LYS	TRP