BMRB Entry 5027
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5027
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Niimi, Tatsuya; Osawa, Masanori; Yamaji, Noboru; Yasunaga, Kunio; Sakashita, Hitoshi; Mase, Toshiyasu; Tanaka, Akihiro; Fujita, Shigeo. "NMR Structure of Human Fibronectin EDA" J. Biomol. NMR 21, 281-284 (2001).
PubMed: 11775745
Assembly members:
human fibronectin EDA, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapien
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
human fibronectin EDA: NIDRPKGLAFTDVDVDSIKI
AWESPQGQVSRYRVTYSSPE
DGIHELFPAPDGEEDTAELQ
GLRPGSEYTVSVVALHDDME
SQPLIGTQSTAIPA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 619 |
| 13C chemical shifts | 392 |
| 15N chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human fibronectin EDA | 1 |
Entities:
Entity 1, human fibronectin EDA 94 residues - Formula weight is not available
| 1 | ASN | ILE | ASP | ARG | PRO | LYS | GLY | LEU | ALA | PHE | ||||
| 2 | THR | ASP | VAL | ASP | VAL | ASP | SER | ILE | LYS | ILE | ||||
| 3 | ALA | TRP | GLU | SER | PRO | GLN | GLY | GLN | VAL | SER | ||||
| 4 | ARG | TYR | ARG | VAL | THR | TYR | SER | SER | PRO | GLU | ||||
| 5 | ASP | GLY | ILE | HIS | GLU | LEU | PHE | PRO | ALA | PRO | ||||
| 6 | ASP | GLY | GLU | GLU | ASP | THR | ALA | GLU | LEU | GLN | ||||
| 7 | GLY | LEU | ARG | PRO | GLY | SER | GLU | TYR | THR | VAL | ||||
| 8 | SER | VAL | VAL | ALA | LEU | HIS | ASP | ASP | MET | GLU | ||||
| 9 | SER | GLN | PRO | LEU | ILE | GLY | THR | GLN | SER | THR | ||||
| 10 | ALA | ILE | PRO | ALA |
Samples:
sample_1: human fibronectin EDA, [U-15N; U-13C], 2.0 mM; sodium phosphate 20 mM; Na2SO4 400 mM
cond_set_1: pH: 7.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C CT-HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CA)CO | not available | not available | not available |
| 3D HCACO | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HBHA(CBCACO)NH | not available | not available | not available |
| 3D H(CCO)NH | not available | not available | not available |
| 3D C(CO)NH | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D 13C-edited NOESY-HSQC | not available | not available | not available |
| 3D 15N-edited NOESY-HSQC | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
Software:
NMRpipe - Data processing
PIPP - Peak picking
NMR spectrometers:
- Bruker AMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks