BMRB Entry 50259

Name: 1H, 13C and 15N Resonance Assignments of the Transactivation domain of the Human Forkhead Transcription Factor FOXO4
Published: 2021-08-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50259

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Resonance Assignments of the Transactivation domain of the Human Forkhead Transcription Factor FOXO4

Deposition date:

2020-05-04

Original release date:

2021-08-17

Authors:

Park, Chin-Ju; Ahn, Dabin; Kim, Jinwoo

Citation:

Citation: Kim, Jinwoo; Ahn, Dabin; Park, Chin-Ju. "FOXO4 Transactivation Domain Interaction with Forkhead DNA Binding Domain and Effect on Selective DNA Recognition for Transcription Initiation" J. Mol. Biol. 433, 166808-166808 (2021).
PubMed: 33450250

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 GST TEV LIC cloning vector (2G-T)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DLDLDMYMENLECDMDNIIS DLMDEGEGLDFNFEPDP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	100
15N chemical shifts	34
1H chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FOXO4 TAD	1

Entities:

Entity 1, FOXO4 TAD 37 residues - Formula weight is not available

1

ASP

LEU

ASP

LEU

ASP

MET

TYR

MET

GLU

ASN

2

LEU

GLU

CYS

ASP

MET

ASP

ASN

ILE

SER

3

ASP

LEU

MET

ASP

GLU

GLY

GLU

GLY

LEU

ASP

4

PHE

ASN

PHE

GLU

PRO

ASP

PRO

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; FOXO4 TAD, [U-100% 13C; U-100% 15N], 800 uM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks