BMRB Entry 50258

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50258

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Title:
ch-TOG 1-250
Deposition date:
2020-05-01
Original release date:
2020-12-07
Authors:
Ryan, Veronica; Naik, Mandar; Fawzi, Nicolas
Citation:

Citation: Ryan, Veronica; Perdikari, Theodora; Naik, Mandar; Saueressig, Camillo; Lins, Jeremy; Dignon, Gregory; Mittal, Jeetain; Hart, Anne; Fawzi, Nicolas. "Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration"  EMBO J. 40, e105001-e105001 (2021).
PubMed: 33349959

Assembly members:

Assembly members:
entity_1, polymer, 250 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDDSEWLKLPVDQKCEHKL WKARLSGYEEALKIFQKIKD EKSPEWSKFLGLIKKFVTDS NAVVQLKGLEAALVYVENAH VAGKTTGEVVSGVVSKVFNQ PKAKAKELGIEICLMYIEIE KGEAVQEELLKGLDNKNPKI IVACIETLRKALSEFGSKII LLKPIIKVLPKLFESREKAV RDEAKLIAVEIYRWIRDALR PPLQNINSVQLKELEEEWVK LPTSAPRPTRFLRSQQELEA KLEQQQSAGG

Data sets:
Data typeCount
13C chemical shifts637
15N chemical shifts190
1H chemical shifts190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TOG D11

Entities:

Entity 1, TOG D1 250 residues - Formula weight is not available

1   METGLYASPASPSERGLUTRPLEULYSLEU
2   PROVALASPGLNLYSCYSGLUHISLYSLEU
3   TRPLYSALAARGLEUSERGLYTYRGLUGLU
4   ALALEULYSILEPHEGLNLYSILELYSASP
5   GLULYSSERPROGLUTRPSERLYSPHELEU
6   GLYLEUILELYSLYSPHEVALTHRASPSER
7   ASNALAVALVALGLNLEULYSGLYLEUGLU
8   ALAALALEUVALTYRVALGLUASNALAHIS
9   VALALAGLYLYSTHRTHRGLYGLUVALVAL
10   SERGLYVALVALSERLYSVALPHEASNGLN
11   PROLYSALALYSALALYSGLULEUGLYILE
12   GLUILECYSLEUMETTYRILEGLUILEGLU
13   LYSGLYGLUALAVALGLNGLUGLULEULEU
14   LYSGLYLEUASPASNLYSASNPROLYSILE
15   ILEVALALACYSILEGLUTHRLEUARGLYS
16   ALALEUSERGLUPHEGLYSERLYSILEILE
17   LEULEULYSPROILEILELYSVALLEUPRO
18   LYSLEUPHEGLUSERARGGLULYSALAVAL
19   ARGASPGLUALALYSLEUILEALAVALGLU
20   ILETYRARGTRPILEARGASPALALEUARG
21   PROPROLEUGLNASNILEASNSERVALGLN
22   LEULYSGLULEUGLUGLUGLUTRPVALLYS
23   LEUPROTHRSERALAPROARGPROTHRARG
24   PHELEUARGSERGLNGLNGLULEUGLUALA
25   LYSLEUGLUGLNGLNGLNSERALAGLYGLY

Samples:

sample_1: TOG D1, [U-100% 13C; U-100% 15N; U-80% 2H], 250 uM; DTT 1 mM; D2O, [U-99% 2H], 10%; MES 20 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe - data analysis

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks