BMRB Entry 50256

Name: hCPEB3
Published: 2022-04-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50256

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

hCPEB3

Deposition date:

2020-05-01

Original release date:

2022-04-12

Authors:

Ramirez de Mingo, Daniel; Laurents, Douglas

Citation:

Citation: Ramirez de Mingo, Daniel; Pantoja-Uceda, David; Carrion-Vazquez, Mariano; Laurents, Douglas. "Human CPEB3's Prion-Like Domain: Preferred Conformations and Implications for Memory Consolidation" Mol. Cell ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 450 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLL3.7-syn

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQDDLLMDKSKTQPQPQQQQ RQQQQPQPESSVSEAPSTPL SSETPKPEENSAVPALSPAA APPAPNGPDKMQMESPLLPG LSFHQPPQQPPPPQEPAAPG ASLSPSFGSTWSTGTTNAVE DSFFQGITPVNGTMLFQNFP HHVNPVFGGTFSPQIGLAQT QHHQQPPPPAPAPQPAQPAQ PPQAQPPQQRRSPASPSQAP YAQRSAAAAYGHQPIMTSKP SSSSAVAAAAAAAAASSASS SWNTHQSVNAAWSAPSNPWG GLQAGRDPRRAVGVGVGVGV GVPSPLNPISPLKKPFSSNV IAPPKFPRAAPLTSKSWMED NAFRTDNGNNLLPFQDRSRP YDTFNLHSLENSLMDMIRTD HEPLKGRMGINFHHPGTDNI MALNSRSSLFPFEDAFLDDS HGDQALSSGLSSPTRCQNGE RVERYSRKVFVGGLPPDIDE DEITASFRRF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	1416
15N chemical shifts	441
1H chemical shifts	384

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 450 residues - Formula weight is not available

1	MET	GLN	ASP	ASP	LEU	LEU	MET	ASP	LYS	SER
2	LYS	THR	GLN	PRO	GLN	PRO	GLN	GLN	GLN	GLN
3	ARG	GLN	GLN	GLN	GLN	PRO	GLN	PRO	GLU	SER
4	SER	VAL	SER	GLU	ALA	PRO	SER	THR	PRO	LEU
5	SER	SER	GLU	THR	PRO	LYS	PRO	GLU	GLU	ASN
6	SER	ALA	VAL	PRO	ALA	LEU	SER	PRO	ALA	ALA
7	ALA	PRO	PRO	ALA	PRO	ASN	GLY	PRO	ASP	LYS
8	MET	GLN	MET	GLU	SER	PRO	LEU	LEU	PRO	GLY
9	LEU	SER	PHE	HIS	GLN	PRO	PRO	GLN	GLN	PRO
10	PRO	PRO	PRO	GLN	GLU	PRO	ALA	ALA	PRO	GLY
11	ALA	SER	LEU	SER	PRO	SER	PHE	GLY	SER	THR
12	TRP	SER	THR	GLY	THR	THR	ASN	ALA	VAL	GLU
13	ASP	SER	PHE	PHE	GLN	GLY	ILE	THR	PRO	VAL
14	ASN	GLY	THR	MET	LEU	PHE	GLN	ASN	PHE	PRO
15	HIS	HIS	VAL	ASN	PRO	VAL	PHE	GLY	GLY	THR
16	PHE	SER	PRO	GLN	ILE	GLY	LEU	ALA	GLN	THR
17	GLN	HIS	HIS	GLN	GLN	PRO	PRO	PRO	PRO	ALA
18	PRO	ALA	PRO	GLN	PRO	ALA	GLN	PRO	ALA	GLN
19	PRO	PRO	GLN	ALA	GLN	PRO	PRO	GLN	GLN	ARG
20	ARG	SER	PRO	ALA	SER	PRO	SER	GLN	ALA	PRO
21	TYR	ALA	GLN	ARG	SER	ALA	ALA	ALA	ALA	TYR
22	GLY	HIS	GLN	PRO	ILE	MET	THR	SER	LYS	PRO
23	SER	SER	SER	SER	ALA	VAL	ALA	ALA	ALA	ALA
24	ALA	ALA	ALA	ALA	ALA	SER	SER	ALA	SER	SER
25	SER	TRP	ASN	THR	HIS	GLN	SER	VAL	ASN	ALA
26	ALA	TRP	SER	ALA	PRO	SER	ASN	PRO	TRP	GLY
27	GLY	LEU	GLN	ALA	GLY	ARG	ASP	PRO	ARG	ARG
28	ALA	VAL	GLY	VAL	GLY	VAL	GLY	VAL	GLY	VAL
29	GLY	VAL	PRO	SER	PRO	LEU	ASN	PRO	ILE	SER
30	PRO	LEU	LYS	LYS	PRO	PHE	SER	SER	ASN	VAL
31	ILE	ALA	PRO	PRO	LYS	PHE	PRO	ARG	ALA	ALA
32	PRO	LEU	THR	SER	LYS	SER	TRP	MET	GLU	ASP
33	ASN	ALA	PHE	ARG	THR	ASP	ASN	GLY	ASN	ASN
34	LEU	LEU	PRO	PHE	GLN	ASP	ARG	SER	ARG	PRO
35	TYR	ASP	THR	PHE	ASN	LEU	HIS	SER	LEU	GLU
36	ASN	SER	LEU	MET	ASP	MET	ILE	ARG	THR	ASP
37	HIS	GLU	PRO	LEU	LYS	GLY	ARG	MET	GLY	ILE
38	ASN	PHE	HIS	HIS	PRO	GLY	THR	ASP	ASN	ILE
39	MET	ALA	LEU	ASN	SER	ARG	SER	SER	LEU	PHE
40	PRO	PHE	GLU	ASP	ALA	PHE	LEU	ASP	ASP	SER
41	HIS	GLY	ASP	GLN	ALA	LEU	SER	SER	GLY	LEU
42	SER	SER	PRO	THR	ARG	CYS	GLN	ASN	GLY	GLU
43	ARG	VAL	GLU	ARG	TYR	SER	ARG	LYS	VAL	PHE
44	VAL	GLY	GLY	LEU	PRO	PRO	ASP	ILE	ASP	GLU
45	ASP	GLU	ILE	THR	ALA	SER	PHE	ARG	ARG	PHE

Samples:

sample_1: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM

sample_2: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 0.4 ± 0.1 mM

sample_3: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM

sample_4: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM

sample_5: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM

sample_6: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 0.6 ± 0.1 mM

sample_7: hCPEB3 IDR, [U-98% 13C; U-98% 15N], 0.6 ± 0.1 mM

sample_conditions_1: ionic strength: 0.0010 M; pH: 4.0; pressure: 0.93 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_1	isotropic	sample_conditions_1
1D 1H	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
1H-15N heteronoe	sample_2	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_2	isotropic	sample_conditions_1
1D 1H	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
1H-15N heteronoe	sample_3	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_3	isotropic	sample_conditions_1
1D 1H	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_4	isotropic	sample_conditions_1
3D HNCO	sample_4	isotropic	sample_conditions_1
1H-15N heteronoe	sample_4	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_4	isotropic	sample_conditions_1
3D HNHA	sample_4	isotropic	sample_conditions_1
1D 1H	sample_5	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_5	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_5	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_5	isotropic	sample_conditions_1
1H-15N heteronoe	sample_5	isotropic	sample_conditions_1
1D 1H	sample_6	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_6	isotropic	sample_conditions_1
3D HNCO	sample_6	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_6	isotropic	sample_conditions_1
1H-15N heteronoe	sample_6	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_6	isotropic	sample_conditions_1
1D 1H	sample_7	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_1
3D HNCO	sample_7	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_7	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_7	isotropic	sample_conditions_1
1H-15N heteronoe	sample_7	isotropic	sample_conditions_1

Software:

SPARKY v3.12 - chemical shift assignment

TOPSPIN v2.1 - collection

NMRPipe - data analysis

TALOS+ - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLN	ASP	ASP	LEU	LEU	MET	ASP	LYS	SER
2	LYS	THR	GLN	PRO	GLN	PRO	GLN	GLN	GLN	GLN
3	ARG	GLN	GLN	GLN	GLN	PRO	GLN	PRO	GLU	SER
4	SER	VAL	SER	GLU	ALA	PRO	SER	THR	PRO	LEU
5	SER	SER	GLU	THR	PRO	LYS	PRO	GLU	GLU	ASN
6	SER	ALA	VAL	PRO	ALA	LEU	SER	PRO	ALA	ALA
7	ALA	PRO	PRO	ALA	PRO	ASN	GLY	PRO	ASP	LYS
8	MET	GLN	MET	GLU	SER	PRO	LEU	LEU	PRO	GLY
9	LEU	SER	PHE	HIS	GLN	PRO	PRO	GLN	GLN	PRO
10	PRO	PRO	PRO	GLN	GLU	PRO	ALA	ALA	PRO	GLY
11	ALA	SER	LEU	SER	PRO	SER	PHE	GLY	SER	THR
12	TRP	SER	THR	GLY	THR	THR	ASN	ALA	VAL	GLU
13	ASP	SER	PHE	PHE	GLN	GLY	ILE	THR	PRO	VAL
14	ASN	GLY	THR	MET	LEU	PHE	GLN	ASN	PHE	PRO
15	HIS	HIS	VAL	ASN	PRO	VAL	PHE	GLY	GLY	THR
16	PHE	SER	PRO	GLN	ILE	GLY	LEU	ALA	GLN	THR
17	GLN	HIS	HIS	GLN	GLN	PRO	PRO	PRO	PRO	ALA
18	PRO	ALA	PRO	GLN	PRO	ALA	GLN	PRO	ALA	GLN
19	PRO	PRO	GLN	ALA	GLN	PRO	PRO	GLN	GLN	ARG
20	ARG	SER	PRO	ALA	SER	PRO	SER	GLN	ALA	PRO
21	TYR	ALA	GLN	ARG	SER	ALA	ALA	ALA	ALA	TYR
22	GLY	HIS	GLN	PRO	ILE	MET	THR	SER	LYS	PRO
23	SER	SER	SER	SER	ALA	VAL	ALA	ALA	ALA	ALA
24	ALA	ALA	ALA	ALA	ALA	SER	SER	ALA	SER	SER
25	SER	TRP	ASN	THR	HIS	GLN	SER	VAL	ASN	ALA
26	ALA	TRP	SER	ALA	PRO	SER	ASN	PRO	TRP	GLY
27	GLY	LEU	GLN	ALA	GLY	ARG	ASP	PRO	ARG	ARG
28	ALA	VAL	GLY	VAL	GLY	VAL	GLY	VAL	GLY	VAL
29	GLY	VAL	PRO	SER	PRO	LEU	ASN	PRO	ILE	SER
30	PRO	LEU	LYS	LYS	PRO	PHE	SER	SER	ASN	VAL
31	ILE	ALA	PRO	PRO	LYS	PHE	PRO	ARG	ALA	ALA
32	PRO	LEU	THR	SER	LYS	SER	TRP	MET	GLU	ASP
33	ASN	ALA	PHE	ARG	THR	ASP	ASN	GLY	ASN	ASN
34	LEU	LEU	PRO	PHE	GLN	ASP	ARG	SER	ARG	PRO
35	TYR	ASP	THR	PHE	ASN	LEU	HIS	SER	LEU	GLU
36	ASN	SER	LEU	MET	ASP	MET	ILE	ARG	THR	ASP
37	HIS	GLU	PRO	LEU	LYS	GLY	ARG	MET	GLY	ILE
38	ASN	PHE	HIS	HIS	PRO	GLY	THR	ASP	ASN	ILE
39	MET	ALA	LEU	ASN	SER	ARG	SER	SER	LEU	PHE
40	PRO	PHE	GLU	ASP	ALA	PHE	LEU	ASP	ASP	SER
41	HIS	GLY	ASP	GLN	ALA	LEU	SER	SER	GLY	LEU
42	SER	SER	PRO	THR	ARG	CYS	GLN	ASN	GLY	GLU
43	ARG	VAL	GLU	ARG	TYR	SER	ARG	LYS	VAL	PHE
44	VAL	GLY	GLY	LEU	PRO	PRO	ASP	ILE	ASP	GLU
45	ASP	GLU	ILE	THR	ALA	SER	PHE	ARG	ARG	PHE

1	MET	GLN	ASP	ASP	LEU	LEU	MET	ASP	LYS	SER
2	LYS	THR	GLN	PRO	GLN	PRO	GLN	GLN	GLN	GLN
3	ARG	GLN	GLN	GLN	GLN	PRO	GLN	PRO	GLU	SER
4	SER	VAL	SER	GLU	ALA	PRO	SER	THR	PRO	LEU
5	SER	SER	GLU	THR	PRO	LYS	PRO	GLU	GLU	ASN
6	SER	ALA	VAL	PRO	ALA	LEU	SER	PRO	ALA	ALA
7	ALA	PRO	PRO	ALA	PRO	ASN	GLY	PRO	ASP	LYS
8	MET	GLN	MET	GLU	SER	PRO	LEU	LEU	PRO	GLY
9	LEU	SER	PHE	HIS	GLN	PRO	PRO	GLN	GLN	PRO
10	PRO	PRO	PRO	GLN	GLU	PRO	ALA	ALA	PRO	GLY
11	ALA	SER	LEU	SER	PRO	SER	PHE	GLY	SER	THR
12	TRP	SER	THR	GLY	THR	THR	ASN	ALA	VAL	GLU
13	ASP	SER	PHE	PHE	GLN	GLY	ILE	THR	PRO	VAL
14	ASN	GLY	THR	MET	LEU	PHE	GLN	ASN	PHE	PRO
15	HIS	HIS	VAL	ASN	PRO	VAL	PHE	GLY	GLY	THR
16	PHE	SER	PRO	GLN	ILE	GLY	LEU	ALA	GLN	THR
17	GLN	HIS	HIS	GLN	GLN	PRO	PRO	PRO	PRO	ALA
18	PRO	ALA	PRO	GLN	PRO	ALA	GLN	PRO	ALA	GLN
19	PRO	PRO	GLN	ALA	GLN	PRO	PRO	GLN	GLN	ARG
20	ARG	SER	PRO	ALA	SER	PRO	SER	GLN	ALA	PRO
21	TYR	ALA	GLN	ARG	SER	ALA	ALA	ALA	ALA	TYR
22	GLY	HIS	GLN	PRO	ILE	MET	THR	SER	LYS	PRO
23	SER	SER	SER	SER	ALA	VAL	ALA	ALA	ALA	ALA
24	ALA	ALA	ALA	ALA	ALA	SER	SER	ALA	SER	SER
25	SER	TRP	ASN	THR	HIS	GLN	SER	VAL	ASN	ALA
26	ALA	TRP	SER	ALA	PRO	SER	ASN	PRO	TRP	GLY
27	GLY	LEU	GLN	ALA	GLY	ARG	ASP	PRO	ARG	ARG
28	ALA	VAL	GLY	VAL	GLY	VAL	GLY	VAL	GLY	VAL
29	GLY	VAL	PRO	SER	PRO	LEU	ASN	PRO	ILE	SER
30	PRO	LEU	LYS	LYS	PRO	PHE	SER	SER	ASN	VAL
31	ILE	ALA	PRO	PRO	LYS	PHE	PRO	ARG	ALA	ALA
32	PRO	LEU	THR	SER	LYS	SER	TRP	MET	GLU	ASP
33	ASN	ALA	PHE	ARG	THR	ASP	ASN	GLY	ASN	ASN
34	LEU	LEU	PRO	PHE	GLN	ASP	ARG	SER	ARG	PRO
35	TYR	ASP	THR	PHE	ASN	LEU	HIS	SER	LEU	GLU
36	ASN	SER	LEU	MET	ASP	MET	ILE	ARG	THR	ASP
37	HIS	GLU	PRO	LEU	LYS	GLY	ARG	MET	GLY	ILE
38	ASN	PHE	HIS	HIS	PRO	GLY	THR	ASP	ASN	ILE
39	MET	ALA	LEU	ASN	SER	ARG	SER	SER	LEU	PHE
40	PRO	PHE	GLU	ASP	ALA	PHE	LEU	ASP	ASP	SER
41	HIS	GLY	ASP	GLN	ALA	LEU	SER	SER	GLY	LEU
42	SER	SER	PRO	THR	ARG	CYS	GLN	ASN	GLY	GLU
43	ARG	VAL	GLU	ARG	TYR	SER	ARG	LYS	VAL	PHE
44	VAL	GLY	GLY	LEU	PRO	PRO	ASP	ILE	ASP	GLU
45	ASP	GLU	ILE	THR	ALA	SER	PHE	ARG	ARG	PHE

1	MET	GLN	ASP	ASP	LEU	LEU	MET	ASP	LYS	SER
2	LYS	THR	GLN	PRO	GLN	PRO	GLN	GLN	GLN	GLN
3	ARG	GLN	GLN	GLN	GLN	PRO	GLN	PRO	GLU	SER
4	SER	VAL	SER	GLU	ALA	PRO	SER	THR	PRO	LEU
5	SER	SER	GLU	THR	PRO	LYS	PRO	GLU	GLU	ASN
6	SER	ALA	VAL	PRO	ALA	LEU	SER	PRO	ALA	ALA
7	ALA	PRO	PRO	ALA	PRO	ASN	GLY	PRO	ASP	LYS
8	MET	GLN	MET	GLU	SER	PRO	LEU	LEU	PRO	GLY
9	LEU	SER	PHE	HIS	GLN	PRO	PRO	GLN	GLN	PRO
10	PRO	PRO	PRO	GLN	GLU	PRO	ALA	ALA	PRO	GLY
11	ALA	SER	LEU	SER	PRO	SER	PHE	GLY	SER	THR
12	TRP	SER	THR	GLY	THR	THR	ASN	ALA	VAL	GLU
13	ASP	SER	PHE	PHE	GLN	GLY	ILE	THR	PRO	VAL
14	ASN	GLY	THR	MET	LEU	PHE	GLN	ASN	PHE	PRO
15	HIS	HIS	VAL	ASN	PRO	VAL	PHE	GLY	GLY	THR
16	PHE	SER	PRO	GLN	ILE	GLY	LEU	ALA	GLN	THR
17	GLN	HIS	HIS	GLN	GLN	PRO	PRO	PRO	PRO	ALA
18	PRO	ALA	PRO	GLN	PRO	ALA	GLN	PRO	ALA	GLN
19	PRO	PRO	GLN	ALA	GLN	PRO	PRO	GLN	GLN	ARG
20	ARG	SER	PRO	ALA	SER	PRO	SER	GLN	ALA	PRO
21	TYR	ALA	GLN	ARG	SER	ALA	ALA	ALA	ALA	TYR
22	GLY	HIS	GLN	PRO	ILE	MET	THR	SER	LYS	PRO
23	SER	SER	SER	SER	ALA	VAL	ALA	ALA	ALA	ALA
24	ALA	ALA	ALA	ALA	ALA	SER	SER	ALA	SER	SER
25	SER	TRP	ASN	THR	HIS	GLN	SER	VAL	ASN	ALA
26	ALA	TRP	SER	ALA	PRO	SER	ASN	PRO	TRP	GLY
27	GLY	LEU	GLN	ALA	GLY	ARG	ASP	PRO	ARG	ARG
28	ALA	VAL	GLY	VAL	GLY	VAL	GLY	VAL	GLY	VAL
29	GLY	VAL	PRO	SER	PRO	LEU	ASN	PRO	ILE	SER
30	PRO	LEU	LYS	LYS	PRO	PHE	SER	SER	ASN	VAL
31	ILE	ALA	PRO	PRO	LYS	PHE	PRO	ARG	ALA	ALA
32	PRO	LEU	THR	SER	LYS	SER	TRP	MET	GLU	ASP
33	ASN	ALA	PHE	ARG	THR	ASP	ASN	GLY	ASN	ASN
34	LEU	LEU	PRO	PHE	GLN	ASP	ARG	SER	ARG	PRO
35	TYR	ASP	THR	PHE	ASN	LEU	HIS	SER	LEU	GLU
36	ASN	SER	LEU	MET	ASP	MET	ILE	ARG	THR	ASP
37	HIS	GLU	PRO	LEU	LYS	GLY	ARG	MET	GLY	ILE
38	ASN	PHE	HIS	HIS	PRO	GLY	THR	ASP	ASN	ILE
39	MET	ALA	LEU	ASN	SER	ARG	SER	SER	LEU	PHE
40	PRO	PHE	GLU	ASP	ALA	PHE	LEU	ASP	ASP	SER
41	HIS	GLY	ASP	GLN	ALA	LEU	SER	SER	GLY	LEU
42	SER	SER	PRO	THR	ARG	CYS	GLN	ASN	GLY	GLU
43	ARG	VAL	GLU	ARG	TYR	SER	ARG	LYS	VAL	PHE
44	VAL	GLY	GLY	LEU	PRO	PRO	ASP	ILE	ASP	GLU
45	ASP	GLU	ILE	THR	ALA	SER	PHE	ARG	ARG	PHE