BMRB Entry 50250

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50250

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Title:
Backbone assignments of LeishIF4E-1 bound to m7GTP
Deposition date:
2020-04-25
Original release date:
2020-09-18
Authors:
Belfetmi, Anissa; Leger-Abraham, Melissa
Citation:

Citation: Belfetmi, Anissa; Leger-Abraham, Melissa. "1H, 13C, and 15N backbone chemical shift assignments of m7GTP cap-bound Leishmania initiation factor 4E-1"  Biomol. NMR Assign. 14, 259-263 (2020).
PubMed: 32519295

Assembly members:

Assembly members:
entity_1, polymer, 214 residues, Formula weight is not available
7-METHYL-GUANOSINE-5'-TRIPHOSPHATE, non-polymer, 538.215 Da.

Natural source:

Natural source:   Common Name: Leishmania major   Taxonomy ID: 5664   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Leishmania major

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSAPSSVPPHKMANLHKLQR AWTLWYDSPSTYNTENWEMS LVPIMTVHSVEEFFVMLRYM KPLHALRTSSQYHFFQEGVK PMWEDPANKKGGKLWVNLDI TSANGRSSNNNTSGTSAADG SAAEAKTDLDKAWENVLMAT VGEYLDCVDKKDTPTEPFVT GIVMSKRKYHNRLAVWVSDA SATDKIEALKKALTKEASLA PIASMVFTKHGEAS

Data sets:
Data typeCount
13C chemical shifts555
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein1
2m7GTP2

Entities:

Entity 1, Protein 214 residues - Formula weight is not available

1   METSERALAPROSERSERVALPROPROHIS
2   LYSMETALAASNLEUHISLYSLEUGLNARG
3   ALATRPTHRLEUTRPTYRASPSERPROSER
4   THRTYRASNTHRGLUASNTRPGLUMETSER
5   LEUVALPROILEMETTHRVALHISSERVAL
6   GLUGLUPHEPHEVALMETLEUARGTYRMET
7   LYSPROLEUHISALALEUARGTHRSERSER
8   GLNTYRHISPHEPHEGLNGLUGLYVALLYS
9   PROMETTRPGLUASPPROALAASNLYSLYS
10   GLYGLYLYSLEUTRPVALASNLEUASPILE
11   THRSERALAASNGLYARGSERSERASNASN
12   ASNTHRSERGLYTHRSERALAALAASPGLY
13   SERALAALAGLUALALYSTHRASPLEUASP
14   LYSALATRPGLUASNVALLEUMETALATHR
15   VALGLYGLUTYRLEUASPCYSVALASPLYS
16   LYSASPTHRPROTHRGLUPROPHEVALTHR
17   GLYILEVALMETSERLYSARGLYSTYRHIS
18   ASNARGLEUALAVALTRPVALSERASPALA
19   SERALATHRASPLYSILEGLUALALEULYS
20   LYSALALEUTHRLYSGLUALASERLEUALA
21   PROILEALASERMETVALPHETHRLYSHIS
22   GLYGLUALASER

Entity 2, m7GTP - C11 H19 N5 O14 P3 - 538.215 Da.

1   MGP

Samples:

sample_1: LeishIF4E-1, [U-100% 13C; U-100% 15N], 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_2: LeishIF4E-1, [U-15N]-Ala, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_3: LeishIF4E-1, [U-100% 13C; U-100% 15N], 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_4: LeishIF4E-1, [U-2H; U-15N; U-13; ILV 13C/1H], 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_5: LeishIF4E-1, [U-15N]-His, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_6: LeishIF4E-1, [U-15N]-Leu, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_7: LeishIF4E-1, [U-15N]-Lys, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_8: LeishIF4E-1, [U-15N]-Gly, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_9: LeishIF4E-1, [U-15N]-Thr, 400 uM; 7-Methyl-GTP 400 uM; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1
3D HCCONHsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_8isotropicsample_conditions_1
2D 1H-15N HSQCsample_7isotropicsample_conditions_1
2D 1H-15N HSQCsample_9isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.3 - chemical shift assignment

CARA v1.9.0 - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

NMRDraw - processing

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks