BMRB Entry 50242
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50242
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Citation: Andralojc, Witold; Pasternak, Karol; Sarzynska, Joanna; Zielinska, Karolina; Kierzek, Ryszard; Gdaniec, Zofia. "The origin of the high stability of 3'-terminal uridine tetrads: contributions of hydrogen bonding, stacking interactions, and steric factors evaluated using modified oligonucleotide analogs" RNA 26, 2000-2016 (2020).
PubMed: 32967936
Assembly members:
entity_1, polymer, 7 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: UGGUGGX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 52 |
| 31P chemical shifts | 6 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | [r(UGGUGG)d(U)]4 G-quadruplex | 1 |
Entities:
Entity 1, [r(UGGUGG)d(U)]4 G-quadruplex 7 residues - Formula weight is not available
| 1 | U | G | G | U | G | G | DU |
Samples:
sample_1: [r(UGGUGG)d(U)]4 G-quadruplex 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-31H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker Avance III 700 MHz