BMRB Entry 50238

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50238

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Title:
Backbone (1H, 13C and 15N) Chemical Shift Assignments and 15N Relaxation Parameters for protein kinase Inhibitor alpha (PKIa) free state
Deposition date:
2020-04-17
Original release date:
2020-05-15
Authors:
Olivieri, Cristina; Li, Geoffrey; Veliparambil Subrahmanian, Manu; Veglia, Gianluigi
Citation:

Citation: Olivieri, Cristina; Wang, Yingjie; Li, Geoffrey; Veliparambil Subrahmanian, Manu; Kim, Jonggul; Stultz, Benjamin; Neibergall, Matthew; Porcelli, Fernando; Muretta, Joseph; Thomas, David; Gao, Jiali; Blumenthal, Donald; Taylor, Susan; Veglia, Gianluigi. "Multi-state recognition pathway of the intrinsically disordered protein kinase inhibitor by protein kinase A"  eLIFE 9, e55607-e55607 (2020).
PubMed: 32338601

Assembly members:

Assembly members:
entity_1, polymer, 75 residues, 7857.33 Da.

Natural source:

Natural source:   Common Name: rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TDVETTYADFIASGRTGRRN AIHDILVSSASGNSNELALK LAGLDINKTEGEEDAQRSST EQSGEAQGEAAKSES

Data sets:
Data typeCount
13C chemical shifts208
15N chemical shifts74
1H chemical shifts425
T1 relaxation values73
T2 relaxation values73
heteronuclear NOE values70

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PKIA1

Entities:

Entity 1, PKIA 75 residues - 7857.33 Da.

1   THRASPVALGLUTHRTHRTYRALAASPPHE
2   ILEALASERGLYARGTHRGLYARGARGASN
3   ALAILEHISASPILELEUVALSERSERALA
4   SERGLYASNSERASNGLULEUALALEULYS
5   LEUALAGLYLEUASPILEASNLYSTHRGLU
6   GLYGLUGLUASPALAGLNARGSERSERTHR
7   GLUGLNSERGLYGLUALAGLNGLYGLUALA
8   ALALYSSERGLUSER

Related Database Links:

UNP P61926
AlphaFold P04541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks