BMRB Entry 50234

Title:
15N-spin relaxation data for TRBP2-dsRBD1 dynamics in presence of D10-RNA
Deposition date:
2020-04-14
Original release date:
2022-02-16
Authors:
Paithankar, Harshad; Chugh, Jeetender
Citation:

Citation: Paithankar, Harshad; Tarang, Guneet Singh; Parvez, Firdousi; Marathe, Aniket; Joshi, Manali; Chugh, Jeetender. "Relay of intrinsic microsecond timescale conformational dynamics observed in an RNA-binding domain of TRBP upon enthalpy-driven binding of dsRNA"  Biophys. J. ., .-. (2022).
PubMed: 35134335

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available
entity_2, polymer, 10 residues, Formula weight is not available
entity_3, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSV272

Data sets:
Data typeCount
T1 relaxation values58
T2 relaxation values58
heteronuclear NOE values58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRBP2-dsRBD11
2D10-RNA, chain 12
3D10-RNA, chain 23

Entities:

Entity 1, TRBP2-dsRBD1 108 residues - Formula weight is not available

N-terminal residues GGA are non-native to the protein and are left after the cleavage of the fusion tag using protease.

1   GLYGLYALAMETSERGLUGLUGLUGLNGLY
2   SERGLYTHRTHRTHRGLYCYSGLYLEUPRO
3   SERILEGLUGLNMETLEUALAALAASNPRO
4   GLYLYSTHRPROILESERLEULEUGLNGLU
5   TYRGLYTHRARGILEGLYLYSTHRPROVAL
6   TYRASPLEULEULYSALAGLUGLYGLNALA
7   HISGLNPROASNPHETHRPHEARGVALTHR
8   VALGLYASPTHRSERCYSTHRGLYGLNGLY
9   PROSERLYSLYSALAALALYSHISLYSALA
10   ALAGLUVALALALEULYSHISLEULYSGLY
11   GLYSERMETLEUGLUPROALALEU

Entity 2, D10-RNA, chain 1 10 residues - Formula weight is not available

5'-UUAUAAAUGC-3'

1   UUAUAAAUGC

Entity 3, D10-RNA, chain 2 10 residues - Formula weight is not available

5'-GCAUUUAUGA-3'

1   GCAUUUAUGA

Samples:

sample_1: TRBP2-dsRBD1, [U-100% 15N], 200 uM; D10-RNA, chain 1 100 uM; D10-RNA, chain 2 100 uM; D2O, [U-2H], 10%; sodium phosphate 10 mM; sodium chloride 10 mM; EDTA 1 mM; DTT 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMRPipe - processing

NMRFAM-SPARKY - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz