BMRB Entry 5023

Title:
Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter   PubMed: 11273704
Deposition date:
2001-05-21
Original release date:
2001-08-20
Authors:
Tinoco, I.; Kim, C.-H.; Kao, C.
Citation:

Citation: Tinoco, I.; Kim, C.-H.. "Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter"  J. Mol. Biol. 307, 827-839 (2001).

Assembly members:

Assembly members:
mutant BMV (+) strand RNA, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: .

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mutant BMV (+) strand RNA: GGUGCUUAGCACC

Data sets:
Data typeCount
1H chemical shifts10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3')1

Entities:

Entity 1, RNA (5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3') 13 residues - Formula weight is not available

1   GGUGCUUAGC
2   ACC

Samples:

sample_1: mutant BMV (+) strand RNA 2 mM; sodium phosphate buffer 5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 5 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

FELIX v95 - data analysis

X-PLOR v3.1 - refinement, structure solution

NMR spectrometers:

  • Bruker AMX 600 MHz