BMRB Entry 5023
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5023
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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RDF gzip file.
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Citation: Tinoco, I.; Kim, C.-H.. "Structural and thermodynamic studies on mutant RNA motifs that impair the
specificity between a viral replicase and its promoter" J. Mol. Biol. 307, 827-839 (2001).
PubMed: 11273704
Assembly members:
mutant BMV (+) strand RNA, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: .
Entity Sequences (FASTA):
mutant BMV (+) strand RNA: GGUGCUUAGCACC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 10 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA (5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3') | 1 |
Entities:
Entity 1, RNA (5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3') 13 residues - Formula weight is not available
| 1 | G | G | U | G | C | U | U | A | G | C | ||||
| 2 | A | C | C |
Samples:
sample_1: mutant BMV (+) strand RNA 2 mM; sodium phosphate buffer 5 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 5 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
Software:
FELIX v95 - data analysis
X-PLOR v3.1 - refinement, structure solution
NMR spectrometers:
- Bruker AMX 600 MHz