BMRB Entry 50229

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50229

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Title:
1H, 13C and 15N resonance assignments for the microtubule-binding region of the kinetoplastid kinetochore protein KKT4 115-343
Deposition date:
2020-04-09
Original release date:
2020-07-24
Authors:
Ludzia, Patryk; Akiyoshi, Bungo; Redfield, Christina
Citation:

Citation: Ludzia, Patryk; Akiyoshi, Bungo; Redfield, Christina. "1 H, 13 C and 15 N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei"  Biomol. NMR Assignments 14, 309-315 (2020).
PubMed: 32696260

Assembly members:

Assembly members:
entity_1, polymer, 231 residues, 25701.57 Da.

Natural source:

Natural source:   Common Name: Trypanosoma brucei brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF_Duet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMKYGVVSVERYERLMARYK ELEKQSHRRQGKRSEPVVDT QRVLDLEEEVARLKRTIGHL QGVVEEKESALEKHATQHNL EVHEMKKNYELKIKSLTQTH EAAVRKLVSAQELVTAARNY QTAVCANNVGGGNSVSTTSG QPLSNTVNHTRGLTTTSSGS GPNQPYTLPHPDGNAWMSAT TSDDRSAPVTTKNSHSVKRE REGTVSTTPTRPLKKRNPRT PSYTVADRISE

Data sets:
Data typeCount
13C chemical shifts393
15N chemical shifts134
1H chemical shifts523

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1KKT4 115-3431

Entities:

Entity 1, KKT4 115-343 231 residues - 25701.57 Da.

Residues 1-2 (113S, 114M) represent part of a linker that remained after TEV protease cleavage.

1   SERMETLYSTYRGLYVALVALSERVALGLU
2   ARGTYRGLUARGLEUMETALAARGTYRLYS
3   GLULEUGLULYSGLNSERHISARGARGGLN
4   GLYLYSARGSERGLUPROVALVALASPTHR
5   GLNARGVALLEUASPLEUGLUGLUGLUVAL
6   ALAARGLEULYSARGTHRILEGLYHISLEU
7   GLNGLYVALVALGLUGLULYSGLUSERALA
8   LEUGLULYSHISALATHRGLNHISASNLEU
9   GLUVALHISGLUMETLYSLYSASNTYRGLU
10   LEULYSILELYSSERLEUTHRGLNTHRHIS
11   GLUALAALAVALARGLYSLEUVALSERALA
12   GLNGLULEUVALTHRALAALAARGASNTYR
13   GLNTHRALAVALCYSALAASNASNVALGLY
14   GLYGLYASNSERVALSERTHRTHRSERGLY
15   GLNPROLEUSERASNTHRVALASNHISTHR
16   ARGGLYLEUTHRTHRTHRSERSERGLYSER
17   GLYPROASNGLNPROTYRTHRLEUPROHIS
18   PROASPGLYASNALATRPMETSERALATHR
19   THRSERASPASPARGSERALAPROVALTHR
20   THRLYSASNSERHISSERVALLYSARGGLU
21   ARGGLUGLYTHRVALSERTHRTHRPROTHR
22   ARGPROLEULYSLYSARGASNPROARGTHR
23   PROSERTYRTHRVALALAASPARGILESER
24   GLU

Related Database Links:

UniProt A0A3L6L4L8
AlphaFold A0A3L6L4L8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks